摘要
为研究致病性大肠杆菌对河北省南都规模化鸡舍空气的污染情况及其耐药性,根据细菌分离培养和形态学特性、生化试验、动物试验、玻板凝集试验分离鉴定致病性大肠杆菌,并应用K-B纸片法测定其对12种抗菌药的耐药性。结果显示,共分离鉴定出26株致病性大肠杆菌,其中从1个试验鸡舍最多能分离到5株致病性大肠杆菌;共分离出7个血清型,O78占所分离致病菌株的比例最高;致病性大肠杆菌对抗菌药的敏感性不同,对头孢类药物的敏感性高,对氟哌酸、多西环素、卡那霉素的敏感性低。表明鸡舍空气受大肠杆菌的污染程度不同,不同鸡舍分离大肠杆菌的耐药性和血清型差异较大。
In order to research the contamination state of pathogenic E. coli in chicken house air of Hebei province and observe its resistance to antibiotics. Pathogenic E. coli was isolated and identified by the characteristics of bacteria cultivation, morphology, biochemistry experiment, animal experiment and glass board agglutinate experiment. The resistance to 12 antibiotics was detected with K-B method. The results showed that 26 pathogenic E. coli were isolated,5 strains were isolated from the same chicken house;7 serotype were identified,and 078 took up the highest proportion. The sensitivity to the antibiotics were different. The highly sensitive antibiotics was cephalosporin drugs. The low sensitive antibiotics was norfloxacin, doxycycline and kanamycin. The results indicated that the contamination state of pathogenic E. coli,the resistance to antibiotics and the serotype of separated pathogenic E. coli were different.
出处
《河南农业科学》
CSCD
北大核心
2014年第2期126-128,共3页
Journal of Henan Agricultural Sciences
基金
河北省人力资源和社会保障厅资助项目(2012-26)
关键词
大肠杆菌
耐药性
空气
鸡舍
Escherichia coli
resistance
air
chicken house
