摘要
【目的】建立同时测定金黄膏中盐酸小檗碱、巴马丁含量的方法。【方法】采用高效液相色谱法，色谱柱为Apollo C18（250mm×4．6mm，5μm），流动相为乙腈-0．1％磷酸（二元梯度洗脱），检测波长为346nm，流速为1．0mL／min，柱温为30℃。【结果】盐酸小檗碱和巴马丁的进样量分别在0．00725-0．145μg，0．0055-0．110μg范围内与各自峰面积积分值呈良好的线性关系，平均加样回收率分别为98．8％和100．9％。【结论】该方法操作简便、重现性好，适用于金黄膏中巴马丁和盐酸小檗碱的含量测定。
[Objective] To establish a method for simultaneous determination of berberine and palmatine in linhuang cream. [Methods] A HPLC method was performed on Apollo CIS （250mm×4.6mm,5μm） column. The mobile phase consisted of acetonitrile-0. 1 % phosphoric acid （gradient elution ）with the detection wavelengh at 346 nm. The flow rate was 1.0 mUmin and the column temperature was at 30 ℃. [Results] The content of berberine hydrochloride in the range of 0.00725-0.145 μg and the content of palmatine in the range of 0.005 5- 0.110 μg had good linear relationships with the area of eluted peak, and the recovery rate （the average recoveries） was 98.8% and 100.9% respectively. [Conclusion] This method is simple and repeatable, and is suitable for the content determination of berberine and palmatine in Iinhuang cream.
出处
《天津中医药》
CAS
2014年第2期110-112,共3页
Tianjin Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(81072818)
