摘要
目的建立测定乌蕨中牡荆苷含量的高效液相色谱方法。方法采用DIONEX-C18柱(4.6mm×250mm,5μm),以乙腈∶甲醇∶0.2%冰醋酸水溶液(23∶77)为流动相,流速1.0mL/min,柱温30℃,检测波长270nm。结果牡荆苷在0.009-0.180mg/mL浓度范围内有良好线性关系(r=0.9995),平均加样回收率为98.0%,RSD小于2.0%。结论本方法简便、快速、准确、重复性好,可用于乌蕨中牡荆苷的含量测定。
Objective To establish a HPLC method for the determination of vitexin in Stenoloma chusanum （ L inn. ） Ching. Method The HPLC system consisted of the DIONEX - C18 （4.6mm × 250mm, 5μm） column, the mobile phase consisted of acetonitrile - water （23 ： 77） with 0.2% glacial acetic, the flow rate was 1.0mL/min, the column temperature was 30℃, The UV detector was set at 270rim. Results The linear response range was 0. 009 - 0. 180mg/mL（ r = 0.9998）. The average recovery of mangiferin was 98.0%, and RSD 〈 2%. Conclusion The method is simple, rapid, accurate and repeatable. It can be applied in determination of Vitexin in Stenoloma chusa- num. it can provide evidence for further development and utilization of this crude drug.
出处
《湖北中医药大学学报》
2014年第1期47-49,共3页
Journal of Hubei University of Chinese Medicine
关键词
乌蕨
牡荆苷
高效液相色谱法
Stenoloma chusanum （ Linn. ） Ching.
Vitexin
HPLC
