摘要
[目的]建立不同产地丹参5种脂溶性成分二氢丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ、1，2-二氢丹参酮的含量测定方法，比较不同产地丹参脂溶性成分含量的差异。[方法]以不同产地丹参为材料，用HPLC法测定，色谱柱为Agilent ZORBAX Eclipse Plus C18柱（Analytial 4．6×250mm．5μn），以乙腈-水为流动相进行梯度洗脱，检测波长为270ntn，柱温为25℃，进样量为20μl。[结果]二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、1，2-二氢丹参酮、丹参酮ⅡA的线性范围分别为0．0099～0．9900、0．0207～3．1050、0．0196～1．9600、0．0217～2．1700、0．0414～4．1400μg／ml（R。分别为0．9998、0．9999、0．9996、0．9998和0．9998），线性关系良好；平均回收率分别为99．92％、100．15％、100．10％、99．96％和100．34％；RSD分别为0．957％、1．175％、1．038％、1．156％和1．04％。不同产地丹参中5种成分含量差别较大。[结论]该方法稳定可靠，重现性好，可用于丹参中脂溶性成分的测定。
[ Objective ] To establish the method for the content determination of dihydrotanshinone I, tanshinone II A, cryptotanshinone, tan-shinone Ⅰ, 1,2-dihydrotanshinone in Salviae miltiorrhizae from different habitats. [ Method ] HPLC method was adopted. The determination was performed on Agilent ZORBAX Eclipse Plus CIs （ Analytial 4.6 × 250 mm, 5 μm） column with the gradient eluent consisted of acetonitrile-wa-ter. The detection wavelength was set at 270 nm and the column temperature was 25℃. The injection size was 20 μL. [ Result] The linear ranges of dihydrotanshinone Ⅰ, tanshinone ⅡA, cryptotanshinone, tanshinone Ⅰ, 1,2-dihydrotanshinone in S. miltiorrhizae were 0. 009 9 - 0.99001xg （R2=0.9998）,0.0207-3.1050μg（R^2=0.9999）,0.0196-1.9600~g（R2=0.9996）,0.0217-2.1700μg（R^2= 0.999 8）, 0.041 4 - 4. 140 0μg （ R^2 = 0. 999 8）, respectively. The average recoveries were 99.92%, 100.15%, 100.10%, 99.96%, 100.34%, respectively, RSDs were 0.957%, 1. 175%, 1. 038%, 1. 156%, 1.04% （ n = 6）. There were significant differences in the con-tents of dibydrotanshinone I, tanshinone II A, cryptotanshinone, tanshinone Ⅰ, 1,2-dihydrotansbinone in S. miltiorrhizae from different habi-tats. [ Conclusion] The method is stable, reliable and reproducible for the quality control of S. miltiorrhizae.
出处
《安徽农业科学》
CAS
2013年第32期12575-12577,12579,共4页
Journal of Anhui Agricultural Sciences
