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长期酒精摄入对雄性大鼠生殖系统的损伤 被引量:2

Chronic Alcohol Intake Contributes to the Injury in Reproductive System of Male Rats
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摘要 目的:研究长期酒精摄入对雄性大鼠生殖系统的损伤机制。方法:选用8周龄的SD大鼠,进行随机分组:对照组(5%蔗糖,口服);酒精组(4g/kg,口服)。连续12周后,分别取附睾考察精子数目、活力;取血清检测睾酮和促黄体生产素(LH)含量;计算睾丸-体重比,并检测睾丸中丙二醛(MDA)、谷胱甘肽(GSH)含量以及谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)的活性;同时检测凋亡相关蛋白bax,bcl-2以及caspase-3前体和剪切体的蛋白表达。结果:酒精组12周后,大鼠的睾丸-体重比明显降低(P<0.05),精子数目减少(P<0.01),精子活力下降(P<0.01);血清中睾酮含量下降(P<0.05),LH含量增加(P<0.05);睾丸中MDA含量增加(P<0.01),GSH含量降低(P<0.05),GPx和SOD活性下降(P<0.01);凋亡相关蛋白bax表达增加(P<0.05),caspase-3剪切体与前体的比值增加(P<0.01)。结论:长期摄入酒精引起的大鼠睾丸内氧化应激水平的增加是其导致其生殖系统损伤的重要因素之一。 Objective: To investigate the mechanism of chronic alcohol intake contributed to the injury in reproductive system of male rats. Methods: Male SD rats, aged 8 weeks old, were randomly divided into two groups and treated as follows: (i) control (5% sucro- se, orally) daily for 12 weeks, (ii) ethanol (4 g/kg, orally) daily for 12 weeks. 12 weeks later, epididymises were removed and analyzed for sperm number and motility. Serum was isolated and analyzed for concentration of testosterone and LH. Testis were removed and content of MDA and GSH, activity of GPx and SOD, expression of bax, bcl-2, caspase-3 and cleaved caspase-3 were analyzed. Results: 12 weeks later, the ratio of testicular weight and body weight decreased in ethanol-treated group (P〈0.05). The number and motility of sperm decreased (P〈0.01), and the concentration of testosterone and LH in serum decreased (P〈0.05) and increased (P〈0.05), respectively. The content ofMDA (P〈0.01) and GSH (P〈0.05) increased. The activity of GSH and GPx decreased (P〈0.01). The expression ofbax in- creased(P〈0.05) and the ratio of cleaved caspase-3 and caspase-3 elevated (P〈0.01). Conclusion: The augmentation of oxidative stress contributes to the ethanol-induced reproductive injury in male rats.
机构地区 解放军第
出处 《现代生物医学进展》 CAS 2014年第5期858-861,869,共5页 Progress in Modern Biomedicine
关键词 酒精 氧化应激水平 雄性生殖系统损伤 Ethanol Oxidative stress Male reproductive system
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  • 1Theodore J. Cicero, Brace Nock, Lynn O'Connor, et al. Acute alcohol exposure markedly influences male fertility and fetal outcome in the male rat [J]. Life Sci, 1994, 55:901-901.
  • 2Yoshishige Ida, Shusaku Tsujimam, Katsura Nakamaura, et al. Effects of acute and repeated alcohol ingestion on hypothalamic-pituitary-gon- adal and hypothalamic-pituitary-adrenal functioning in normal males [J]. Drug Alcohol Depend, 1992, 31:57-57.
  • 3Qianlong Zhu, Jeremy Meisinger, Nicholas V. Emanuele, et al. Ethan- ol exposure enhances apoptosis within the testes [J]. Alcohol Clin Exp Res, 2000, 10:1550-1556.
  • 4E1-Sokkary GH. Quantiative study on the effects of chronic ethanol ad- ministration on the testis of adult male rats [J]. Neuro endocrinol Lett, 2001, 22:93-99.
  • 5Arto K. Orpana, C.J.Peter Eriksson, Matti H~irk~inen. Ethanol- induced inhibition of testosterone biosynthesis in rat leydig cells; role of cultu- re medium composition [J]. J Steroid Biochem, 1989, 33:1243-1248.
  • 6Tom V. Widenius, C.J.Peter Eriksson, Reino H. Ylikahri, et al.lnhibi-tion of testoterone synthesis by ethanol:role of luteinizing hormone [J]. Alcohol, 1989, 6:241-241.
  • 7Ellingboe J. Acute effects of ethanol on sex hormones in non-alcoholic men and women [J]. Alcohol and Alcoholism. Supplement 1987, 1: 109-116.
  • 8王水明,彭瑞云,高亚兵,常公民,姚斌伟,王少霞,赵黎,李杨,徐新萍.极短期微波辐射对雄性大鼠生殖系统的影响[J].军事医学,2011,35(5):375-378. 被引量:8
  • 9罗琼,任世成,杨明亮,闫俊,杨佳娟.海带多糖对睾丸受辐射雄性大鼠性功能的影响[J].营养学报,2009,31(3):259-262. 被引量:13
  • 10高海燕,李杰,司纪亮,安丽红.妊娠期二月桂酸二丁基锡暴露对子代雄性大鼠生殖系统的影响[J].环境与健康杂志,2010,27(11):952-954. 被引量:1

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