摘要
目的:探讨电针"足三里"穴调控急性胰腺炎大鼠肠道炎性反应的作用机制。方法:将54只雄性SD大鼠随机分为重症急性胰腺炎(SAP)模型组、电针组、假手术组,每组18只。通过逆行胆胰管注射3.5%牛磺胆酸钠制作SAP模型。模型制作成功后电针组在双侧"足三里"穴给予电针治疗30min。假手术组及模型组大鼠在同一时间固定30min不予治疗。各组大鼠均于造模后3h、6h、12h分批处死,观察胰腺以及小肠病理学改变,并用免疫组化SP法检测小肠上皮组织闭锁蛋白(occludin protein)以及核因子-κB(NF-κB p65)表达水平。结果:各时间点胰腺病理评分假手术组、电针组均低于模型组(P<0.05);小肠上皮组织occludin蛋白表达假手术组与电针组均高于模型组(P<0.05);小肠上皮组织NF-κB p65表达假手术组与电针组均低于模型组(P<0.05)。结论:电针"足三里"穴可以降低SAP模型大鼠小肠上皮组织NF-κB p65表达,提高小肠上皮组织occludin蛋白表达,从而减轻胰腺损伤。
Objective To explore the mechanism of electroacupuncture at Zusanli （ST 36） on regulation of in- testinal inflammatory reaction in rats with acute pancreatitis. Methods Fifty-four SD rats were randomly divided into a severe acute pancreatitis （SAP） group, an electroacupuncture （EA） group and a sham-operation （SO） group, 18 cases in each group. 3. 5% sodium cholate was used to made SAP model by retrograde injecting m cholangiopancreatic duct. After the success of model making, the EA group was treated with EA at bilateral Zu- sanli （ST 36） for 30 min. The SO group and the SAP group were fixed at the same time for 30 rain without treat- ment. All the rats were killed at 3 h, 6 h and 12 h after modeling in batches. The pathological changes of pancreat- ic tissue and intestinal epithelium were observed, and the expression of small intestinal occludin protein and nuclear factor kappa-B （NF-κB） were detected by immunohistochemical SP method. Results The pathologic score and the expression of small intestinal NF-κB p65 at 3 h, 6 h and 12 h after modeling in the EA group and the SO group were significantly lower than those in the SAP group （all P〈0.05）, and the expression of small intestinal occludin protein in the EA group and the SO group were significantly higher than that in the SAP group （all P〈0.05）. Conclusion Electroacupuncture at Zusanli （ST 36） can alleviate pancreatic injury by reducing the expression of NF-κB p65 and enhancing the expression of occludin protein in the intestinal epithelium in the SAP model rats.
出处
《中国针灸》
CAS
CSCD
北大核心
2014年第3期267-271,共5页
Chinese Acupuncture & Moxibustion
基金
卫生部临床重点专科基金资助项目:97050234
