摘要
目的研究抗菌肽LL-37对鲍曼不动杆菌生物膜的影响,为将来在临床感染的控制和新抗菌药物的研发提供科学的依据。方法运用平板结合结晶紫染色法体外建立鲍曼不动杆菌生物膜模型,肉汤稀释法检测LL-37的最小抑菌浓度;采用扫描电镜和结晶紫染色定量法观察抗菌肽LL-37作用后生物膜结构和量的改变。结果成功体外建立鲍曼不动杆菌生物膜模型,测得抗菌肽LL-37 MIC为64μg/ml;经过抗菌肽LL-37作用后,鲍曼不动杆菌成熟生物膜结构被破坏。当LL-37浓度为2.5μg/ml时即可破坏生物膜结构,且随着LL-37浓度增加,生物膜的量逐渐减少。结论抗菌肽LL-37在浓度远小于其MIC时即可破坏鲍曼不动杆菌生物膜结构。
Objective To investigate the effect of antibacterial peptide LL-37 on the integrity of Acinetobacter baumannii biofilm. Methods A model of Acinetobacter baumannii biofilm in vitro was constructed by plates and crystal violet staining method, and the minimal inhibitory concentration of LL-37 was determined by broth dilution. The biofilm morphology was observed by scanning electron microscopy and biofilm formation was analyzed by the crystal violet staining of the adherent biofilm in the presence of antibacterial peptide LL-37. Results In the Acinetobacter baumannii biofilm model, the minimal inhibitory concentration of LL-37 was 64μg/ml;LL-37 caused structural damage of the biofilm at a low concentration of 2.5μg/ml. The biofilm was decreased gradually as the concentration of LL-37 increased. Conclusion LL-37 even at a concentration far below its minimal inhibitory concentration can cause structural damage of Acinetobacter baumannii biofilm in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2014年第3期426-429,共4页
Journal of Southern Medical University
基金
广东省自然科学基金(9151051501000067)
