摘要
比较了抗癌结构类似物吗丙嗪和雷佐生对红细胞膜钙调蛋白 (CaM)活性的影响 .观察兔红细胞膜Ca2 +,Mg2 + ATP酶 (CaM的靶酶 )活性反映膜CaM激活ATP酶的活性 .结果显示吗丙嗪 ( 0 .1～ 1mmol·L- 1)浓度依赖性抑制CaM激活ATP酶活性 (下降11%～ 32 % ) .雷佐生在高浓度 ( 0 .5mmol·L- 1)仍未显示抑制CaM激活ATP酶活性的作用 .吗丙嗪在低浓度 ( 0 .0 2～ 0 .1mmol·L- 1)能增加N 乙酰神经氨酸( 0 .2mmol·L- 1)抑制CaM激活ATP酶活性的作用 .结果表明吗丙嗪对CaM活性的抑制作用强于雷佐生 ,因而可能在CaM介导的抑瘤途径的作用较雷佐生强 ,且其作用可能与神经氨酸 (唾液酸 )
To compare the effects of antitumor derivatives probimane and razoxane on calmodulin(CaM) in rabbit erythrocytes by observing CaM activated Ca 2+ ,Mg 2+ ATPase activity of rabbit erythrocyte membranes. It was showed that probimane (0.1-1.0 mmol·L -1 ) inhibited cell CaM activated Ca 2+ ,Mg 2+ ATPase activity (decreasing 11%-32%) in a dose dependent manner. Razoxane at a concentration of 0.5 mmol·L -1 did not inhibit CaM activated Ca 2+ ,Mg 2+ ATPase activity. Probimane at low concentrations (0.02-0.1 mmol·L -1 ) enhanced the inhibitory effect of N acetylneuraminic acid on CaM activated Ca 2+ ,Mg 2+ ATPase activity. The results manifest that probimane may be more potent to CaM pathways of tumor than razoxane does and its effect might be correlated with neuraminic acids(sialic acids).
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
2001年第1期76-78,共3页
Chinese Journal of Pharmacology and Toxicology
基金
上海市高等教育科学技术发展基金资助项目!(97A49)&&
