摘要
目的研究杜仲叶总提取物对去势大鼠骨质疏松(osteoporosis,OP)模型的骨代谢调节作用机制。方法 4月龄雌性SD大鼠32只,随机分成正常对照组(Control组,生理盐水灌胃)、去卵巢对照组(OP组,生理盐水灌胃)、雌二醇组(Estrogen组,雌二醇水溶液灌胃)、杜仲叶总提取物组(DZ组,杜仲叶总提物水溶液灌胃)。各组治疗3个月后,通过眼眶采血,测定血清中Ca2+、P3+和ALPase活力。并取大鼠的股骨和胫骨,用双能X线骨密度仪测定骨密度。结果血清中Ca2+、P3+和ALPase活力,DZ组与OP组比较,均有显著差异(P<0.05);股骨和胫骨BMD,DZ组与OP组比较,有显著差异(P<0.05)。结论杜仲叶总提取物可以改善OP模型动物骨代谢,增加骨质疏松模型动物的骨密度,减少骨破坏,加强骨稳定,有效防治骨质疏松症。
Objective Study of adjusting effect of total extraction in Eucommia ulmoides oliv leaf on bone metabolism in os-teoporosis （OP） model animal’s OP and its mechanism. Methods Thirty-two SD female rats at the age of 4 months were randomly divided into 4 groups：normal control group （gavage with physiological saline）,ovariectomized group （OP group,gavage with physio-logical saline）,estrogen group （gavage with estrogen）,total extraction in Eucommia ulmoides oliv. Leaf group （DZ group,gavage with total extraction of Eucommia ulmoides oliv. leaf）. At the end of 3-month treatment,blood calcium （Ca2＋）,blood phosphor （P3＋）, and alkaline phosphates （ALP） were tested by orbital blood collection. Thon femur and tibia of 4-group animals were taken out. Bone mineral density （BMD） of them was determined using dual-energy X-ray absorptiometry. Results Compared with OP group, blood calcium （Ca2＋）,blood phosphor （P3＋）,and alkaline phosphatase （ALP） in DZ group were decreased significantly （P〈0.05）;bone mineral density of femurs and tibia in DZ group were remarkably increased （P〈0.05）. Conclusion Total extraction in Eu-commia ulmoides oliv. leaf can improve bone metabolism of OP animals,increase BMD,prevent and treat osteoporosis effectively.
出处
《江西医药》
CAS
2014年第2期100-102,共3页
Jiangxi Medical Journal
基金
国家自然基金资助项目(30460160)
关键词
杜仲叶总提物
骨质疏松症
动物模型
骨密度
Total extraction in Eucommia ulmoides oliv leaf
Osteoporosis
Animal model
Bone mineral density
