摘要
利用同源克隆法从新疆无苞芥中克隆获得1个锌指蛋白基因(OpZFP)。序列分析表明,OpZFP基因的开放阅读框为684bp,推测编码含227个氨基酸的蛋白质。生物信息学分析显示,OpZFP蛋白含有1个典型的C2H2型锌指结构,在C端含有一个可能具有转录抑制功能的EAR结构域。系统进化树分析表明OpZFP编码产物与拟南芥AtZFP1、琴叶拟南芥AlZFP1的进化关系较近。分离了OpZFP基因2 095bp的启动子序列,发现该启动子与拟南芥AtZFP1基因的启动子序列只有84.4%的相似性,启动子分析表明二者存在多处不同的顺式作用元件。半定量RT-PCR分析表明,OpZFP在根、茎、叶、花和果荚中均有表达,在根中的表达量最高。OpZFP基因受高盐、干旱和低温等胁迫的诱导表达,表明该蛋白涉及多种胁迫相关的信号传导途径。
Zinc finger protein plays important roles in plant stress tolerance. OpZFP, A zinc finger protein gene of Olirnarabidopsis pumila ,was isolated by homologous sequence cloning method. The open reading frame of OpZFP gene was 684 bp,encoding 227 amino acids which containing a typical Cys2/His2 zinc fin- ger domain,an EAR-motif at its C-terminal region. Phylogenetic analysis revealed that OpZFP had a closer relationship with AtZFP1 and A1ZFP1. A OpZFP promoter sequence covering 2 095 bp upstream of tran- scription start site was isolated from O. pumila,and showed only 84.4~ similarity with that of AtZFP1 gene promoter. Several different cis-elements were found analyzed in two promoters. Expression analysis by semi-quantitative RT-PCR indicated that OpZFP displayed a much broader expression range in different tissues in O. pumila,with a maximum expression in root. OpZFP gene was induced by high salt, drought and low temperature, suggesting that the function of OpZFP might involve in some stress related signal pathways.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第2期243-250,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31060149)
新世纪优秀人才支持计划(NCET-12-1072)
石河子大学高层次人才启动项目(RCZX200902)
石河子大学SRP项目(SRP20130067)
关键词
无苞芥
锌指蛋白
ZFP基因
逆境
Olimarabidopsis pumila
zinc finger protein
ZFP gene ~ stress
