摘要
采用大孔吸附树脂分离纯化人工蛹虫草培养基残基中的虫草素,以HPLC法测定样品中虫草素含量,筛选出了适宜的大孔树脂NKA-II。研究了pH、洗脱剂乙醇体积分数等因素对该树脂吸附性能及解析性能的影响。结果表明,NKA-II型树脂纯化虫草素的最佳吸附条件为pH9,吸附流速2BV/h,该树脂对虫草素的吸附量可达到16.5mg/g。洗脱工艺条件为2.5倍树脂柱体积的50%乙醇,NKA-II大孔树脂对虫草素的解析率可达到95%以上。
Seperating cordycepin from cordycep manual medium residues by macroporous resin, Filter out a suitable macroporous resin NKA-II with HPLC. pH, eluent concentration of ethanol and other factors were researched to the influence of absorbance and desorption of the resin. When the pH value is 9, Adsorption velocity is 2BV/h, the resin has better absorption capacity which reach 16. 5mg/g. When the eluent concentration of ethanol is 50% and the volume is 2.5 times of the resin bed, the resin has better desorption caoacitv which can reach 95%.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2014年第1期90-94,共5页
China Biotechnology
关键词
虫草素
大孔树脂
分离纯化
Cordycepin Macroporous resin Purification
