摘要
目的研究血清25-羟-维生素D(25(OH)D)水平及维生素D受体(VDR)基因多态性与宫颈癌发生发展的关系,探讨25(OH)D及VDR基因多态性在宫颈癌早期诊断中的应用价值,并为进一步研究维生素D是否可以作为宫颈癌预防与治疗的手段奠定基础。方法收集2010年1月-2011年12月期间因宫颈疾病来我院就诊且具备活检病理和LEEP病理结果的患者共179例,收集血清标本应用ELISA方法检测25(OH)D水平;同时收集全血标本应用PCRLDR法检测VDR基因rs11568820(Cdx-2),rs1544410(BsmI),rs7975232(ApaI),rs731236(TaqI)多态性。结果血清25(OH)D水平在各组患者之间无差别(P>0.05);各等位基因频率分布在各组患者之间无差别(P>0.05);宫颈癌和HSIL患者rs7975232(Apa I)CC纯合子的比例显著高于LSIL和慢性宫颈炎患者。结论患者25(OH)D水平与宫颈癌发生无显著相关性;VDR rs7975232(Apa I)CC基因型是高级别宫颈病变(HSIL、宫颈癌)发生的独立风险因素之一。
Objective To study relationship between serum 25 （OH） D level, vitamin D receptor（VDR） gene polymorphism and incidence of cervical cancer, so as to investigate the value of 25 （OH） D and VDR gene polymorphism in the early diagno- sis of cervical cancer, and lay the foundation for further study on whether vitamin D can be used in prevention and treatment of cervical cancer. Methods Serum specimens of 179 cervical diseases patients with biopsy and LEEP pathology results in the hospital from January 2010 to December 2011 were collected to detect 25 （OH） D level by ELISA, and the whole blood speci- mens were collected to detect the polymorphism of VDR gene rs11568820 （Cdx -2）, rs1544410 （Bsral）, rs7975232 （Apal）, rs731236 （Taql） using PCR - LDR. Results Both serum 25 （OH） D level and alleles frequency distribution had nO signifi- cant difference among patients （P 〉 0.05）. The proportion of rs7975232 （Apal） CC homozygotes was higher in cervical cancer patients and HSIL patients than that of LSIL patients and chronic cervicitis patients. Conclusion There was no significant cor- relation between serum 25 （OH） D level and cervical cancer incidence. VDR rs7975232 （Apal） CC genotype is one of the in- dependent risk factors for high - grade cervical lesions （ HSIL, cervical cancer）.
出处
《中国卫生检验杂志》
北大核心
2014年第4期552-555,共4页
Chinese Journal of Health Laboratory Technology
基金
上海市嘉定区科研课题(2010-12-10)
