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重组人表皮生长因子的分离与纯化的研究

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摘要 本实验采用凝胶法来纯化人表皮生长因子,并定量测定了人表皮生长因子(hEGF)。人表皮生长因子与经过离子交换柱、SDSPAGE及凝胶图象扫描分析系统的标准品相互对比。结果表明,在SDS-PAGE凝胶上能够得到与标准样品hEGF一样清晰的单条带,得到的产品纯度可高于94%,检出限能够达到1ug/mL。本实验方法能够很好的在线检测出人表皮生长因子,同时本方法对其它蛋白质分离的在线检测也具有参考价值。
作者 商安新
出处 《黑龙江科技信息》 2014年第3期23-23,共1页 Heilongjiang Science and Technology Information
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参考文献3

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  • 3童望宇,姚善泾,关怡新,朱自强.基因工程人表皮生长因子的分离纯化及其浓度的在线检测[J].分析测试学报,2004,23(6):22-24. 被引量:1

二级参考文献11

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