摘要
目的探讨STAT3在IL-6刺激肝细胞增殖过程中对C/ebpβ表达变化的影响。方法用含15 ng/ml的IL-6培养基培养大鼠正常肝细胞BRL-3A,运用CCK试剂盒检测该细胞在IL-6刺激后不同时间里的增殖情况,Western blot技术检测STAT3、P-STAT3、C/ebpβ的蛋白表达变化情况;利用siRNA瞬时转染技术抑制STAT3在BRL-3A细胞中的表达并观察IL-6刺激肝细胞增殖过程中C/ebpβ的表达变化。结果 IL-6能有效的刺激肝细胞增殖,在增殖早期STAT3、P-STAT3、C/ebpβ表达均明显上调;在肝细胞增殖过程中沉默STAT3后C/ebpβ表达下调。结论在IL-6肝细胞增殖过程中STAT3对C/ebpβ起正向调控作用。
This study performed to investigate the effect of STAT3 on C/ebpβ expression in hepatocyte proliferation process stimulated by IL-6. We used CCK kits to detect the proliferation of normal rat liver cell BRL- 3A, which were cultured with medium containing 15 ng/ml IL-6 for different times; then Western blot was employed to evaluate the changes of the expression of STAT3, P-STAT3, and C/ebpβ. Subsequently, the expression of STAT3 in BAL-3A cell was suppressed by siRNA transient transfection technique and the change of C/ebpβ expression was observed in the process of hepatocyte proliferation stimulated by IL-6. Data suggested that IL-6 could effectively stimulate the hepatocyte proliferation, and the protein expression of STAT3, P-STAT3, and C/ebpβ were significantly increased in the early stage of hepatocyte proliferation; the expression of C/ebpβ would down-regulated in the process of hepatocyte proliferation when STAT3 silenced. The result indicated that STAT3 has a positive regulatory effect on C/ebpβ in the process of hepatocyte proliferation stimulated by IL-6.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第3期202-205,210,共5页
Immunological Journal
基金
国家自然科学基金面上项目(81270523)
