摘要
目的研究中药NDRF(主药为羌活、独活、防风及木瓜)对佐剂性关节炎兔的抗炎免疫机制。方法佐剂性关节炎模型新西兰白兔分为模型组、中药NDRF组、MTX治疗组、中药NDRF联合MTX治疗组、正常对照组,各组经灌胃或腹腔注射给药。酶联免疫吸附法检测各组肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)、血管内皮生长因子(VEGF)水平。RT-PCR法检测滑膜组织TNF-α、IL-1β、VEGF mRNA的相对表达水平。结果血清TNF-α、IL-1β、VEGF在中药组中含量分别为102.21、2.92、75.15 ng/L,模型组分别为166.73、7.76、218.65 ng/L,中药组与模型组比较差异有统计学意义(P<0.05);联合组分别为41.47、2.61、72.36 ng/L,与模型组比较差异有统计学意义(P<0.05)。滑膜组织TNF-αmRNA、IL-1βmRNA、VEGF mRNA在中药组的相对表达量分别是1.37、0.44、1.05,模型组分别是5.45、3.08、3.14,中药组与模型组比较差异有统计学意义(P<0.05);联合组分别是0.94、0.04、0.53,与模型组比较差异有统计学意义(P<0.01)。结论中药NDRF对佐剂性关节炎新西兰白兔抗炎和免疫调节作用与通过抑制炎症细胞因子分泌和下调mRNA相关。
To evaluate the effects of Chinese medicine NDRF combined with methotrexate （MTT） on rabbits with adjuvant arthritis （AA）, we constructed the AA model with tumor necrosis factor alpha （TNF-α） emulsified in Freund＇s complete adjuvant （CFA）. Fifty rabbits were divided randomly into two groups, with 8 of them in the normal group （group A） and the rest in the model group. The model group was sensitized by TNF-α and CFA through plantar subcutaneous injection, and was boosted once after 10 days. The constructed model rabbits were then randomly divided into the following sub-groups： the control model group （group B）, the Chinese medicine NDRF group （group C）, MTX group （group D）, and the NDRF combined with MTX group （group E）. Groups A and B were intragastrieally treated by 10 ml of saline for each rabbit every day; groups C and E were intragastrically administered NDRF daily in a dose of 1.0 g/kg; groups D and E were administered by NDRF in a same way as group C, meanwhile, both the two groups were intraperitoneally administered with MTX in a dose of 1.0 mg/kg per week. After 35 days, articular synovium of all rabbits was collected for pathological examination; furthermore the expression of TNF-α、IL-1β and VEGF in serum was tested by ELISA and the relative expression level of TNF-α、IL-1β and VEGF mRNA was also tested by RT-PCR. Results showed that rabbits in group E demonstrated remarkable lower expression of TNF-α、IL-1β and VEGF in serum （P 〈 0.05）, and significant lower relative expression of TNF-α、IL-1β and VEGF mRNA in synovial tissue （P 〈 0.01）. Consequently, we conclude that the anti-inflammatory and immunomodulatory effects of Chinese medicine NDRF may be associatedwith suppressing anti-inflammatory cytokines secretion and downregulating the mRNA expression of anti- inflammatory cytokines.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2014年第3期235-239,共5页
Immunological Journal
基金
贵州省免疫学研究生教育创新基地重点培育项目(201201)
