摘要
目的:探索艾灸对基因干扰类风湿性关节炎家兔血清细胞因子IL-17的影响及其抗炎效应的作用机制。方法:日本大耳白兔随机分为空白组、模型组、艾灸组、艾灸+SOCS1基因干扰组(简称SOCS1干扰组)、艾灸+PTPN22基因干扰组(简称PTPN22干扰组)、艾灸+PIAS1基因干扰组(简称PIAS1干扰组),6只/组。弗氏完全佐剂按0.5 mL/Kg剂量平均注入兔双后膝关节腔塑造RA模型,RA造模第2 d将相应siRNA按50μg/只剂量平均注入基因干扰组家兔双后膝关节腔。艾灸组及艾灸+基因干扰组动物采用艾粒施灸两侧"肾俞"穴各5壮,1次/d,6 d为1疗程,共治疗3个疗程。治疗后采用免疫酶联法(Elisa法)严格按操作流程测定各实验组家兔血清IL-17水平。结果:与空白组比较,模型组家兔血清IL-17含量极显著升高(P<0.01);与模型组比较,艾灸组家兔血清IL-17含量有极显著降低(P<0.01);PIAS1干扰组、SOCS1干扰组家兔血清IL-17含量有显著降低(P<0.05),PTPN22干扰组有降低趋势;PTPN22干扰组、PIAS1干扰组、SOCS1干扰组与艾灸组比较,下调IL-17含量效果不及艾灸组。结论:艾灸对实验性RA家兔的抗炎效应可能通过下调细胞因子IL-17含量有关,而SOCS1、PTPN22、PIAS1对细胞因子传递重要通路JAK-STAT通路的良性调控可能是其实现抗炎效应的重要机制之一。
Objective： To explore moxibustion on RNA interference rheumatoid arthritis rabbits＇s serum cytoines IL-17 and its anti-inflammatory effect mechanism. Methods： Japanese white rabbits were randomly divided into control group, model group, moxi- bustion group, moxibustion ＋ SOCS1 gene interference group （referred to SOCS1 interference group）, moxibustion ＋ PTPN22 gene interference group （referred to PTPN22 interference group）, moxibustion ＋ PIAS1 gene interference group （referred to PIAS1 inter- ference group）, n =6. Injected freund＇s complete adjuvant by 0. 5 mL/Kg average into the rabbit＇s double rear knee joint cavity to shape RA model, and on the next day injected siRNA 50p.g / each one average into the gene interference rabbit＇s double rearknee joint cavity. Moxibustion group and moxibustion ＋ gene interference group were treated by moxibustion moxa sides ＂Shen-shu＂ points each five Zhuang. one day, 6 d for a course of treatment , three courses in all Immune ELISA （ Elisa method） were used in strict accord-ante with operating procedures to determine in each experimental group rabbit serum IL-17 levels after the treatment. Results： Com- pared with the control group, model group rabbit serum IL-17 levels were significantly higher （P 〈 0. 01 ）; Compared with model group, moxibustion group rabbit serum IL-17 levels have decreased significantly （P 〈 0. 01 ） ; PIAS1 interference group and SOCS1 interference group serum IL-17 levels were significantly lower （P 〈 0. 05 ）, FTPN22 interference group tended to decrease; PTPN22 interference group, PIASI interference group and SOCS1 interference group reduced IL-17 levels less effective than moxibustion group. Conclusion： Moxibustion＇s anti-inflammatory effects on experimental rabbits may because of reducing cytokine IL-17 levels, whereas SOCS1, PTPN22, PIAS1 benign regulate on important cytokine JAK-STAT pathway may be the inflammatory effect of the im- pertant mechanisms.
出处
《成都中医药大学学报》
2014年第1期24-28,47,共6页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(项目批准号:81001555)
