摘要
试验以低温脱脂葵花籽粕为原料,进行脱脂和脱绿原酸处理后,用碱性蛋白酶制备葵花籽多肽。以水解度和ACE抑制率为评价指标,对底物浓度、加酶量、酶解温度、酶解时间和pH值进行单因素试验,在此基础上采用响应面试验优化碱性蛋白酶制备葵花籽多肽的最佳水解工艺。结果表明:底物浓度为15g/mL、加酶量为3 700U/g、酶解温度为56℃和pH值为8.2的条件下水解3h,ACE抑制率可达63.74%。
Taking low temperature defatted suutlower meal as raw material; sunflower seeds polypeptide was prepared with alkaline protease after degreasing, removed chlorogenic acid treatment. Degree of hydrolysis and ACE inhibitor）, rate as evaluation indexes, effects of substrate concentration, enzyme dosage, enzymolysis time, enzymolysis temperature and pH value were studied with single factor experiment. On this basis, optimum hydrolysis process was investigated wilh response surface method. Results showed that substrate concentration 15g/mL, enzyme dosage 3 700U/g, enzymolysis temperature 56℃, pH value 8.2 and enzymolysis time 3h, ACE inhibitory rate was up to 63.74%.
出处
《粮油加工（电子版）》
2014年第2期36-39,42,共5页
Machinery for Cereals Oil and Food Processing Main Contents
关键词
葵花籽粕
碱性蛋白酶
水解度
ACE抑制率
Sunflower seed meal, Alkaline protease, Degree of hydrolysis ACE inhibitor, rate
