摘要
目的 探讨B7-H4在膀胱癌组织中的表达及其与临床病理和预后的关系.方法 以酶联免疫反应检测2007年3月至2008年3月确诊的30例膀胱癌患者外周血中sB7-H4蛋白的含量,以正常人和膀胱良性肿瘤患者外周血中的sB7-H4含量为对照.以实时荧光定量PCR（real timePCR）检测30例膀胱癌新鲜组织中B7-H4 mRNA相对表达量,以癌旁组织和膀胱良性组织为对照.免疫组化染色法检测70例膀胱癌患者手术切除组织标本中B7-H4的表达水平.结果 sB7-H4蛋白在膀胱癌患者外周血中的含量[（45.25±8.93）μg/L]明显高于膀胱良性肿瘤患者[（34.41 ±6.91） μg/L]和正常人群（31.20±6.28） μg/L（P＜0.01）.B7-H4 mRNA在30例膀胱癌组织和癌旁组织中均有表达,膀胱癌组织中表达明显高于癌旁组织（P＜0.01）,且癌旁组织中的表达明显高于膀胱良性肿瘤组织（P＜0.01）.70例膀胱癌组织中B7-H4蛋白阳性表达率为69％,其中77％为高表达,明显高于膀胱良性肿瘤组织（P＜0.01）.B7-H4蛋白的表达与膀胱癌TNM分期和远处转移密切相关（P＜0.05）,与患者性别、年龄、组织类型、临床分期无关（P＞0.05）.B7-H4高表达患者生存率明显低于低表达患者（P＜0.05）.结论 B7-H4在膀胱癌组织表达状态与膀胱癌的转移及预后关系密切,可以作为反映膀胱癌生物学行为和判断预后的有效指标.
Objective To investigate the expression of B7-H4 and its clinical pathological characteristics in bladder cancer tissue and its relationship with the prognosis in bladder cancer patients.Methods From March 2007 to March 2008,30 patients with bladder cancer were recruited.B7-H4 protein in serum was detected by enzyme immunoassay （ELISA） while the content of B7-H4 protein in the serun of patients with bladder benign tumors and the normal population were detected as control.The expression of B7-H4 mRNA was detected in the fresh tissues of 30 patients with bladder cancer by real time quantitative PCR and the expression of B7-H4 mRNA in the adjacent tissues of bladder cancer and bladder benign tumors were detected as control.The B7-H4 expression levels in the tissue samples of 70 patients with bladder cancer was detected by immunohistochemical staining.Results B7-H4 protein [（45.25±8.93） μg/L] in serum of bladder cancer patients was significantly higher than those with bladder benign tumors [（34.41±6.91）μg/L] as well as the normal tissue [（31.20±6.28）μg/L] （P＜0.01）.B7-H4 mRNA expression in bladder cancer tissue was significantly higher than that of the adjacent tissue of bladder cancer （P＜0.01）,and the expression of B7-H4 mRNA in the adjacent tissues of bladder cancer was significantly higher than that of the bladder benign tumors （P＜0.01）.The positive rate of B7-H4 protein in 70 cases of bladder cancer tissues was 69％,including 77％ highly expressed.The positive rate in bladder cancer patients was significantly higher than that of the bladder benign tissue （P＜0.01）.The expression of B7-H4 protein were significantly correlated with TNM classification and distant metastasis of bladder cancer （P＜0.05）,but no significant difference was detected in term of gender,age,histological type,clinical stage （P＞0.05）.The survival rate of the patients with highly B7-H4 expression was lower than those with low B7-H4 expression （P ＜ 0.05）.Conclusions The expression of B7-H4 in bladder cancer was correlated with the metastasis and prognosis,and B7-H4 could be used as an indicator of the biological behavior of bladder cancer and the prognosis.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2014年第2期143-146,共4页
Chinese Journal of Urology
