摘要
目的研究建立人食管癌裸鼠皮下移植及原位移植模型的方法。方法从新鲜的人食管癌标本上切除肿瘤组织,移植于裸鼠皮下,建立原代移植瘤并连续传代3次后,取肿瘤组织分别移植于裸鼠皮下及食管黏膜下,分别观察移植瘤成瘤率、大体及组织形态、肿瘤的侵袭和转移情况。结果皮下移植模型成瘤率60%,为低分化鳞癌,未见淋巴结及远处器官转移;原位移植模型成瘤率80%,与患者原发肿瘤组织形态一致,可见广泛的淋巴结转移及肝肺转移。结论初步建立了有效的人食管癌裸鼠皮下移植及原位移植模型,为食管癌的研究提供了重要工具。
Objective To establish a subcutaneous and orthotopic transplatation tumor model of human esophageal cancer in nude mice. Methods Fresh tissues of human esophageal cancer were collected and transplanted into subcutaneous places of nude mice. The successfully transplanted primary generation tumor was passed down three times. Then tumor were implanted into the subcutaneous and esophageal submucous tissues of nude mice. The formation rate, morphology, invasion and metastasis of tumor were observed. Results The tumor formation rate of subcutaneous transplatation model was 600% ,it was low grade squamous cell carcinoma without lymph node and distant organ metastasis. The tumor formation rate of orthotopic transplatation model was 80 %, the morphological characteristics were consistent with human original tumor. Lymph node and organ metastasis could be found ex- tensive. Conclusion We preliminary established effective subcutaneous and orthotopic transplatation tumor model of human esopha- geal cancer in nude mice. It might be an available model for further research of esophageal cancer.
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第7期828-829,833,共3页
Chongqing medicine
基金
河南省卫生厅资助课题(2011020089)
关键词
食管肿瘤
肿瘤移植
裸鼠
esophageal
neoplasms transplantation
nude mice
