期刊文献+

大孔吸附树脂对草鱼肽的脱盐作用 被引量:3

Desalination of Grass Carp Peptides with Macroporous Adsorption Resin
下载PDF
导出
摘要 目的:研究DA201-C大孔吸附树脂对草鱼肽的脱盐作用。方法:对脱盐前后草鱼肽的灰分、肽含量、分子质量及氨基酸组成进行分析,采用脱盐率、血管紧张素转化酶(angiotensin I-converting enzyme,ACE)抑制活性、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH)自由基清除率和铁还原抗氧化能力(ferric reducing antioxidant potential,FRAP)为指标考察其脱盐效果。结果:草鱼肽的脱盐率为82.02%,脱盐处理后,肽片段和疏水氨基酸得到有效富集;ACE抑制活性显著增大,半抑制质量浓度(IC50)由0.36 mg/mL变为0.23 mg/mL;抗氧化性增强,其IC50由5.59 mg/mL变为4.48 mg/mL;总抗氧化值由1.018增大到1.261。结论:采用DA201-C大孔吸附树脂对草鱼肽脱盐是一种简便有效的处理方法。 Objective: To investigate the desalination of grass carp peptides (GCP) with macroporous adsorption resin DA201-C. Methods: Ash and peptide contents, molecular weight and amino acid composition of GCP were analyzed before and after desalination. Desalination efficiency, angiotensin I-converting enzyme (ACE) inhibitory activity, 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant potential (FRAP) were used to evaluate how well GCP was desalinated. The results showed that the desalination efficiency of GCP was 82.02%. The peptide fragments and hydrophobic amino acids were enriched in desalinated GCP. Meanwhile, the ACE inhibitory activity was significantly higher than that of native GCP, showing a decrease in IC50 from 0.36 to 0.23 mg/mL, and the antioxidant activity was also increased showing an IC50 of 4.48 mg/mL compared to 5.59 mg/mL for native GCP. The desalination resulted in an increase in the FRAP value of GCP from 1.018 to 1.261. Conclusion: The use of macroporous adsorption resin DA201-C provides a simple and effective method to desalinate GPC.
出处 《食品科学》 EI CAS CSCD 北大核心 2014年第5期33-36,共4页 Food Science
基金 国家高技术研究发展计划(863计划)项目(2010AA023003) 武汉工业学院校研究生创新基金资助项目(2011cx013)
关键词 大孔吸附树脂 草鱼肽 脱盐 macroporous adsorption resin grass carp peptides desalination
  • 相关文献

参考文献25

二级参考文献111

共引文献93

同被引文献18

引证文献3

二级引证文献15

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部