摘要
本文选择常见的典型食源性微生物金黄色葡萄球菌,从食品微生物安全角度出发,对127株葡萄球菌的菌株特性、耐药性与生物被膜生长能力进行研究,包括菌株生化鉴定;PCR扩增葡萄球菌特异16S rRNA与金葡菌特异femA基因,通过对耐药基因mecA和orfX检测确定菌株的耐药特性;最后,运用结晶紫染色法进行生物被膜能力检测。127株菌株包括119株金葡菌(均携带16S rRNA与femA)与8株凝固酶阴性葡萄球菌8株(仅携带16S rRNA)。119株金葡菌中,107株携带mecA基因与orfX基因,为耐甲氧西林金葡球菌(MRSA);8株凝固酶阴性葡萄球菌中,均携带mecA基因。所有菌株均能生成生物被膜,其中能形成强、中等与弱粘附生物被膜能力菌株分别有5(3.9%)、47(37.0%)和75(59.1%)株。金葡菌中耐药性与生物被膜较为普遍,由于食源性微生物形成生物被膜后,具有逃逸常规消毒和杀菌手段的能力,成为食品安全中的潜在隐患。
Staphylococcus aureus （S. aureus） has been long recognized as a common food-borne pathogen in food safety. To study their individuality, drug resistance and biofilm-forming capacity, a total of 127 Staphylococci strains were subject to the investigation. Preliminary identification of Staphylococcus aureus was conducted with a rapid confirmatory latex agglutination test kit and API staph test strips. S.aureus was further confirmed with the detection of specific 16S rRNA and femA genes by PCR. The molecular method was also used for amplification of mecA and orfX elements to identify meticillin-resistence S. aureus （MRSA）. The biofilm-forrning capacity of 127 clinical isolates of S. aureus was tested using a polystyrene 96-well plate format and crystal violet staining. In this experiment, 127 staphylococci were isolated, in which 107 strains were MRSA, 12 strains belonged to meticillin-sensitive S. aureus （MSSA） and 6 strains contributed to methicillin-resistant coagnlase-negative staphylococci （MRCNS）. All staphylococci isolates formed biofilms, and 75 strains （59.1%） belonged to the weak adhesive group. The molecular method was more accurate and rapid for the identification of strains. The majority of the strains formed weak adhesive biofilms and there was no significant relationship between the genotype of drug resistance and biofilm formation ability.
出处
《现代食品科技》
EI
CAS
北大核心
2014年第3期69-75,共7页
Modern Food Science and Technology
基金
国家973计划项目"(2012CB720800)
国家自然科学基金青年基金项目(31201362)
中央高校基本科研业务费面上项目(2012ZM0060)
