摘要
目的:探寻电针对缺血性脑卒中再灌注损害大鼠cAMP反应原件结合蛋白(cAMP-response Element Binding Protein,CREB)表达的影响。方法:将60只健康成年的SD大鼠驯养3 d随机分成假手术组、模型组以及电针组,每组各20只,使用线栓法大脑中动脉闭塞(Middle Cerebral Artery Occlusion,MCAO)对模型组、电针组大鼠进行脑缺血模型制作,缺血2 h后进行再灌注,电针组大鼠接受电针干预,再灌注3 d后将大鼠断头取脑,进行2,3,5-氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色以及图像软件分析观察脑梗死体积,免疫蛋白印迹法(Western blotting)检测CREB蛋白水平,聚合酶链反应(Reverse Transcription-polymerase Chain Reaction,PCR)检测CREB的基因表达水平。结果:TTC及其图像软件分析表明,电针组的SD大鼠脑梗死面积明显小于模型组(P<0.01);电针组的SD大鼠CREB蛋白水平明显高于模型组(P<0.01);电针组的SD大鼠CREB基因水平明显高于模型组(P<0.01)。结论:电针改善脑缺血再灌注损伤可以通过上调CREB实现。
Objective：To explore the impact of electro-acupuncture on the expression of CREB in the ischemia-reperfusion rats .Meth-ods：Sixty healthy adult SD rats which were domesticated for 3 days were randomly divided into the shame group , the model group and the electro-acupuncture group .There were 20 rats in each group .The rats of the model group and the electro-acupuncture group were given the line bolt method （ MCAO） to complete the brain ischemia model , and were reperfused 2 hours after ischemia .The electro-acu-puncture group adopted the intervention of electro-acupuncture .Three days after the reperfusion , all the rats were beheaded and taken brains.The cerebral infarction volume was observed through TTC staining and image analysis , and the protein expression of CREB were detected by protein imprinting method （ Western blotting ） and gene expression of CREB were tested by Real-Time PCR.Results：TTC and the image analysis showed that the cerebral infarction volume of electro -acupuncture group was significantly smaller than that of the model group （P＆lt;0.01）;the CREB protein level in the electro-acupuncture group was obviously higher than that of the model group （P＆lt;0.01）;the CREB gene level in the electro-acupuncture group was much higher than that of the model group （P＆lt;0.01）.Conclu-sion：Electro-acupuncture can realize the improvement of the cerebral ischemia reperfusion injury by up -regulating CREB.
出处
《世界中医药》
CAS
2014年第2期221-223,共3页
World Chinese Medicine
基金
教育部博士点基金博导类(编号:20103519110001)
青年科学基金项目(编号:81102628)
