摘要
目的:研究二脱甲氧基姜黄素对体外培养人肝癌细胞HepG2 Caspase-3蛋白酶活性的作用。方法:MTT法检测二脱甲氧基姜黄素对体外培养人肝癌细胞HepG2的抑制作用;分光光度检测其对Caspase-3酶活性的影响;免疫组化法检测其对人肝癌细胞HepG2 Caspase-3蛋白的表达。结果:二脱甲氧基姜黄素对人肝癌细胞HepG2有明显的抑制作用(P<0.001)且呈剂量关系,与姜黄素有显著差异(P<0.001);二脱甲氧基姜黄素能增强Caspase-3酶活性,增强Caspase-3蛋白的表达。结论:二脱甲氧基姜黄素能激活Caspase-3蛋白活性,这可能也是它体外抑制肝癌细胞HepG2生长的机理之一。
Ojective ： To study the effects of bisdemethyoxycurcumin on the activity of Caspase - 3 in HepG2. Method： MTI＂ was used to observe its effects on anti - proliferation of HepG2. The activity of Caspase - 3 was detected by spectro- photography. The Caspase - 3 protein expression was tested by immunohistochemical method. Result ： The bisdemethyoxy- curcumin could inhibit the growth of HepG2 ceils （P 〈0.001 ） and it was more effective on anti - tumor than curcumin （P 〈 0. 001 ）. It had significant effect on increasing Caspase- 3 expression. Conclusion： The bisdemethyoxycurcumin could inhibit the growth of HepG2. It can increase the expression of protein Caspase - 3. It can increase the activity of Caspase - 3, which may be one of its mechanisms associated with the inhibition of HepG2 cells growth.
出处
《中华中医药学刊》
CAS
2014年第3期493-495,I0003,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家创新训练计划项目(201210344005)
浙江省中医药科技计划项目(2011ZB026)
浙江省大学生科技创新项目暨新苗人才计划项目(2012R410014)
