摘要
目的 :在前期工作的基础上进一步观察人胚胎甲状腺组织中褪黑素 (Mel)受体的存在及其分布。方法 :取人胚胎 (胎龄 6个月 )甲状腺 ,石蜡包埋 ,切片 ,以免疫组化 ABC法及原位杂交方法进行染色。结果 :免疫组化方法显示 ,人胚胎甲状腺细胞的细胞膜、细胞质和细胞核中 Mel受体 m t1 和 MT2 亚型均呈棕褐色阳性染色 ;原位杂交方法显示 mt1 和 MT2 分布于甲状腺细胞的细胞质及细胞核内 ,呈蓝紫色阳性染色。 结论 :人胚胎甲状腺组织中存在褪黑素受体 mt1 和 MT2 亚型 ,分布于甲状腺细胞的细胞膜、细胞质。
Objective: To make it clear whether there exists melatonin receptor in the thyroid of human embryo. Methods: Thyroid was collected and sliced up to be stained with methods of immunohistochemistry and in situ hybridization. Results: The thyroid tissue was positively dyed, melatonin receptor mt 1 and MT 2 were with both immunohistochemistry and in situ hybridization while brown granules deposited in the membrane, plasma and nuclear of the thyroid cell were with the immunohitochemistry. Conclusion: There exists melatonin receptors in human embryo thyroid, either mt 1 or MT 2, and they exist in the membrane, plasma and nuclear.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第1期15-17,共3页
Academic Journal of Second Military Medical University
基金
香港裘槎基金资助项目!(396 6 30 0 8)
