摘要
目的探讨女性解脲脲原体(Ureaplasma urealyticum,Uu)感染与其男性伴侣Uu感染的关系,为临床诊治Uu感染提供依据。方法对2010年2月至2011年3月就诊并排除其他病原体感染的有症状且Uu阳性女性334例,无症状但Uu阳性女性61例和同期男性伴侣395例,分别取宫颈管或阴道、尿道分泌物进行Uu实时荧光定量-聚合酶链反应(real-time fluorescence quantitative-Polymerase Chain Reaction,FQ-PCR)基因检测和PCR反向斑点杂交(PCR-RDB)基因分群检测。结果 395例男性伴侣中,203例(51.39%)经FQ-PCR检测为Uu阳性,其中,有症状和无症状女性的男性伴侣Uu阳性率分别为56.3%(188/334)和24.6%(15/61),差异有统计学意义(χ2=19.50,P<0.01)。基因分群显示,有症状女性感染者生物1群、生物2群和混合群各占一定比例,但以生物1群占优势,而无症状女性感染者绝大部分以生物1群为主,占86.9%(53/61);有症状女性的男性伴侣中,有尿道炎症状者绝大部分(98.7%,147/149)为生物2群和混合群感染,无尿道炎症状者则绝大部分(94.9%,37/39)为生物1群感染;而无症状女性的男性伴侣均无尿道炎症状,感染主要以生物1群为主,占80.0%(12/15)。进一步分析发现,女性感染生物1群时其男性伴侣发生感染的比例较低,而女性感染生物2群和混合群时,其男性伴侣感染同类基因群的比例则高达98.6%(71/72)和73.0%(84/115)。结论无论女性感染生物1群还是生物2群,其男性伴侣感染的主要是生物2群。因此,在女性Uu感染者及其男性伴侣中进行Uu基因分群分析,不但可指导临床制定有针对性的治疗方案,而且对监测Uu的基因变化、追踪传染源、分析流行趋势有重要意义。
Objective To understand the relationship of Ureaplasma urealyticum （Uu） infection between women and their male partners. Methods By excluding other urethral pathogen infection, 334 symptomatic and 61 asymptomatic women infected with Uu and 395 male partners were surveyed from February 2010 to March 2011. The endocervix or vagina, urethral secretions were collected from them respectively. Fluorescent quantitative PCR （FQ-PCR） was conducted to determine the Uu gene expression and PCR reverse dot blot （PCR-RDB） assay was conducted for the genogroup detection of Uu. Results Of 395 male partners, 203 （51.39% ） were infected with Uu. The infection rates of Uu in symptomatic and asymptomatic women were 56. 3% （188/334） and 24. 6% （15/61） respectively （χ2 =19.50, P〈0.01）. PCR-RDB assay revealed that biovar 1 infection, biovar 2 infection and co-infection were found in the symptomatic women, but biovar I infection was predominant. The asymptomatic women were mainly infected with biovar I, accounting for 86.9% （53/61）, In the symptomatic women＇s male partners, the infection of biovar 2 and co-infection were found in those who had urethritis symptoms （98.7%, 147/149） and the infection of biovar 1 was found in those who had no urethritis symptoms （94. 9%, 37/39）. It was also found that all the asymptomatic women＇s male partners had no urethritis symptoms, and most of them were infected with biovar 1 （80. 0% , 12/15）. Further analysis showed that when the women were infected with biovar 1, the infection rate of Uu in their male partners was relatively low, but when women had biovar 2 infection or co-infection, the infection rates in their male partners were as high as 98.6% （71/72） or 73.0% （84/115）. Conclusion Whether the women were infected with biovar I or biovar 2, biovar 2 infection was found in their male partners. Therefore, the genogroup detection of Uu in infected women and their male partners can help physician to make targeted strategies for treatment of Uu infection and track the source of infection.
出处
《疾病监测》
CAS
2014年第1期37-40,共4页
Disease Surveillance
关键词
解脲脲原体
非淋球菌性尿道炎
性传播疾病
Ureaplasma urealyticum
non-gonococcal urethritis
sexually transmitted diseases
