摘要
目的 建立测定连麦通淋颗粒中连翘苷含量的高效液相色谱法.方法 采用WatersC18色谱柱（250 mm×215;4.6mm,5μm）,流动相为乙腈-水（20：80）,流速为1.0 mL/min,检测波长为277 nm,柱温30℃.结果 连翘苷进样量在6～ 36 μg（r =0.999 7）范围内与峰面积呈良好线性关系,平均加样回收率为99.17％,RSD =0.40％（n=6）.结论 该法灵敏度高、操作简便、准确可靠,可用于连麦通淋颗粒中连翘苷的含量测定.
Objective To develop a HPLC method for the determination of phillyrin ciontent in Lianmai Tonglin Granules. Methods The Waters C18 column （250 mm×4.6 mm,5μm） was used with the mobile phase of acetonitrile-water（20：80）at a flow rate of 1.0 mL/min; the wavelength of UV detection was 277 nm, and the column temperature was 30 ℃. Results There was a good linear relationship（ r = 0. 999 7） when the injection volume of phillyrin was 6- 36 μg;the average recovery rate was 99. 17%, RSD = 0. 40% （n = 6）. Conclusion This method is highly sensitive, convenient to operate, accurate and reliable, which can be used to determine the phillyrin content in Lianmai Tonglin Granules.
出处
《中国药业》
CAS
2014年第6期34-35,共2页
China Pharmaceuticals
