摘要
为研究辛酸钠对细胞甘油三酯合成能力及对乳脂合成关键转录调节因子固醇调节元件结合蛋白1（SREBPl）和过氧化物酶体增殖物激活受体γ（PPARγ）表达的影响，以体外培养的奶牛乳腺上皮细胞为模型，采用CASY细胞分析仪检测细胞活力和增殖能力，甘油三酯定量试剂盒检测培养液中甘油三酯浓度，实时荧光定量PCR（qRT—PCR）检测SREBP1和PPARγ相对表达丰度，Westernblot检测SREBP1和PPARγ蛋白相对表达水平。结果显示，与不添加辛酸钠组相比，4．0、8．0、12．0mmol／L的辛酸钠能显著抑制细胞活力和增殖能力；0．5～2．0mmol／L的辛酸钠以浓度依赖方式显著降低培养液中甘油三酯的浓度，且能降低或显著降低SREBP1、PPARγ的表达。表明，辛酸钠能抑制奶牛乳腺上皮细胞甘油三酯合成，并对乳脂合成关键转录调节因子的表达具有抑制作用。
The aim of this study was to investigate the effects of sodium octanoate on triacylglycerol（TAG） synthesis and expression level of key transcriptional regulatory factors SREBP1 and PPARy in milk fat synthesis of dairy cow mammary epithelial cells（DCMECs） cultured in vitro. Cell viability and proliferation ability were measured by CASY-technology. TAG contents in medium were detected by TAG quantitation kit. The mRNA and protein expression level of SREBP1 and PPARy were determined by qRT-PCR and Western blot,respectively. The results showed as follows：compared with the group without sodium octanoate,the cell viability and proliferation ability were significantly inhibited by 4. 0,8.0, 12.0 mmol/L sodium octanoate; the contents of TAG in medium were significantly decreased in concentration-dependent manner from 0. 5 to 2.0 mmol/L sodium octanoate;the expression of SREBP1 and PPAR7 were reduced or significantly reduced by sodium octanoate in concentration-dependent manner from 0. 5 to 2.0 mmol/L. This study suggests that sodium oetanoate can inhibit TAG accumulation and the expression of key transcriptional regulatory factors of milk fat synthesis in DCMECs.
出处
《河南农业科学》
CSCD
北大核心
2014年第3期133-138,共6页
Journal of Henan Agricultural Sciences
基金
黑龙江省教育厅科学技术研究基金项目(12531638)
关键词
辛酸钠
奶牛乳腺上皮细胞
甘油三酯合成
乳脂合成
转录调节因子
sodium octanoate
daily cows mammary epithelial cells
triglyceride synthesis
milk fat synthesis
transcriptional regulatory factor
