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CTRP3对TGF-β_1诱导的血管外膜成纤维细胞增殖及α-SMA表达的影响 被引量:6

Effects of CTRP3 on TGF-β_1 induced proliferation and α-SMA expression in adventitial fibroblasts
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摘要 目的探讨C1q肿瘤坏死因子相关蛋白3(CTRP3)对转化生长因子β1(TGF-β1)诱导的血管外膜成纤维细胞(AFs)增殖以及α-平滑肌肌动蛋白(α-SMA)表达的调节作用。方法采用细胞贴壁法培养SD大鼠胸主动脉AFs;CCK-8检测不同浓度CTRP3(0.1、1、10和50μg/mL)对AFs增殖的影响;免疫荧光、Real-Time PCR和Western blotting检测CTRP3对AFsα-SMA表达的影响。结果 CTRP3可呈剂量依赖性抑制TGF-β1诱导的AFs增殖,随着浓度升高,其抑制能力增强,浓度为10μg/mL时,其对AFs增殖的抑制作用最强(P<0.01);免疫荧光、Real-Time PCR和Western blotting结果显示,CTRP3可明显抑制TGF-β1诱导的α-SMA mRNA和蛋白表达(P<0.01)。结论 CTRP3可在体外抑制TGF-β1诱导AFs的增殖和α-SMA表达,提示其潜在的改善病理性血管重构作用。 Objective To investigate the effects of Clq/TNF-related protein-3 (CTRP3) on transforming growth factor-β1 (TGF--β1) induced proliferation and expression of a-smooth muscle actin (α-SMA) among adventitial fibroblasts (AFs). Methods AFs were isolated from thoracic aortas of male Sprague Dawly (SD) rats. The ceils in passage 3 to 5 were treated with various concentrations of CTRP3 (0. 1, 1, 10, 50 txg/mL). The effects of CTRP3 on proliferation of AFs were examined by Cell Counting Kit (CCK-8). Immunoflueresence assay, Real-Time PCR, and Western blotting were adopted to detect the expression of a-SMA of AFs. Results CTRP3 attenuated the proliferative activities induced by TGF-β1 in a dose-dependent manner, and the most marked effect could be observed at the concentration of 10 μg/mL (P 〈 0. 01). Immunoflueresence assay, Real-Time PCR and Western blotting showed that CTRP3 could significantly inhibited TGF-β1 induced upregulation of a-SMA both at the mRNA and protein levels (P 〈 0.01). Conclusion CTRP3 has significant inhibition effects on proliferation and a-SMA expression of adventitial fibroblasts induced by TGF-β1, which suggests the potential value of CTRP3 in the treatment of pathological vascular remodeling.
出处 《上海交通大学学报(医学版)》 CAS CSCD 北大核心 2014年第3期274-278,共5页 Journal of Shanghai Jiao tong University:Medical Science
基金 上海市科委基金(10JC1408902)~~
关键词 血管外膜成纤维细胞 C1 q肿瘤坏死因子相关蛋白 3 转化生长因子β1 Α-平滑肌肌动蛋白 增殖 adventitial fibroblasts Clq/TNF-related protein-3 transforming growth factor-β1 α-smooth muscle actin proliferation
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  • 1Stenmark KR, Nozik-Grayck E, Gerasimovskaya E. et al. The adventitia: essential role in pulmonary vascular remodeling [ J]. Compr Physiol, 2011 , 1 ( 1 ) : 141 - 161.
  • 2Forte A, Della Corte A, De Feo M, et al. Role of myofibroblasts in vascular remodelling: focus on restenosis and aneurysm [J] . Cardiovasc Res, 2010, 88 (3) : 395 - 405.
  • 3Wildgruber M, Weiss W, Berger H, et al. Association of circulating transforming growth factor beta, tumor necrosis factor alpha and basic fibroblast growth factor with restenosis after transluminal angioplasty [J]. Eur J Vasc Endovasc Surg, 2007, 34 (1) : 35 - 43.
  • 4Schaffler A, Weigert J, Neumeier M, et al. Regulation and function of collagenous repeat containing sequence of 26-kDa protein gene product" cartonectin" [J]. Obesity (Silver Spring), 2007, 15 (2) : 303 - 313.
  • 5Zheng Q, Yuan Y, Yi W, et al. C1q/TNF-related proteins, a family of novel adipokines , induce vascular relaxation through the adiponectin receptor-1/ AMPK/eNOS/nitric oxide signaling pathway [J] . Arterioscler Thromb Vase Biol, 2011,31 (11): 2616 -2623.
  • 6Yi W, Sun Y, Yuan Y, et al. C1q/tumor necrosis factor-related protein-3, a newly identified adipokine, is a novel antiapoptotic, proangiogenic, and cardioprotective molecule in the ischemic mouse heart[J]. Circulation, 2012, 125(25): 3159 -3169.
  • 7Liu H, Lin S, Xiao D, et al. Evaluation of the wound healing potential of Resina Draconis (Dracaena cochinchinensis) in animal models[J]. Evid Based Complement Altemat Med, 2013, 2013: 709865.
  • 8Siow RC, Churchman AT. Adventitial growth factor signalling and vascular remodelling: potential of perivascular gene transfer from the outside-in [J]. Cardiovasc Res, 2007, 75 (4) : 659 - 668.
  • 9Scheffler A, Ehling A, Neumann E, et al. Genomic organization, chromosomal localization and adipocytic expression of the murine gene for CORS-26 (collagenous repeat-containing sequence of 26 kDa protein) [J]. Biochim Biophys Acta, 2003, 1628(1): 64 -70.
  • 10Maeda T, Jikko A, Abe M, et al. Cartducin, a paralog of Acrp30/ adiponectin, is induced during chondrogenic differentiation and promotes proliferation of chondrogenic precursors and chondrocytes [J]. J Cell Physiol, 2006, 206(2): 537 -544.

同被引文献47

  • 1Gordon T, Kannel WB, Hiortland MC,et al. Menopause and coro- nary heart disease. The Framingham Study [ J ]. Ann Intern Med, 1978,89(2) :157 - 161.
  • 2Maeda T, Abe M, Kurisu K, et al. Molecular cloning and charac- terization of a novel gene, CORS26, encoding a putative secretory protein and its possible involvement in skeletal development[ J]. Biol Chem,2001,276(5 ) :3628 - 3634.
  • 3Kopp A, Bala M, Buechler C,et al. C lq/TNF-related protein-3 re- presents a novel and endogenous lipopolysaccharide antagonist of the adipose tissue[ Jl. Endocrinology,2010,151:5267 - 5278.
  • 4Weigert J, Neumeier M, Schaffler A, et al. The adiponectin paral- og CORS-26 has anti-inflammatory properties and is produced by human monocytic cells [ J ]. FEBS Lett,2005,579 : 5565 - 5570.
  • 5Maeda T, Wakisaka S. CTRP3/cartducin is induced by transfor- ming growth factor-betal and promotes vascular smooth muscle cell proliferation[ J]. Cell Biol Int ,2010,3g( 3 ) :261 - 266.
  • 6Yi W, Sun Y, Yuan Y, et al. Clq/tumor necrosis factor-related protein-3, a newly identified adipokine, is a novel antiapoptotic, proangiogenic, and cardioprotective molecule in the ischemic mouse heart[ J]. Circulation ,2012,125 (25) :3159 - 3169.
  • 7Deng W,Li C,Zhang Y,et al. Serum Clq/TNF-related protein-3 (CTRP3) levels are decreased in obesity and hypertension and are negatively correlated with parameters of insulin resistance [ J]. Diabetol Metab Syndr,2015,7:33.
  • 8Choi KM, Hwang SY, Hong HC, et al. Implications of Clq/TNF- related protein-3 ( CTRP-3 ) and progranulin in patients with acute coronary syndrome and stable angina pectoris[ J]. Cardiovasc Di- abeto1,2014, 13 : 14.
  • 9Matthews KA, Kuller LH, Sutton-Tyrrell K, et al. Changes in car- diovascular risk factors during the perimenopause and postmeno- pause and carotid artery atherosclerosis in healthy women [ J ]. Stroke,2001,32(5) :1104 - 1111.
  • 10Croft P, Hannaford PC. Risk factors for acute myocardial infarc- tion in women : evidence from the Royal College of General Practi- tioners' oral contraception study [ J ]. BMJ, 1989,298 (6667) : 165 - 168.

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