摘要
目的:探讨有氧运动和饮食干预对肥胖小鼠血清Visfatin和内脏脂肪、肝脏组织Visfatin mRNA水平的影响,及其与氧化应激(OS)的关系。方法:40只C57BL/6J小鼠随机分为普通膳食对照组(ND组)10只,高脂饮食组(HD组)30只。建立肥胖模型后,再将HD组随机分为:高脂肥胖对照组(OB组)、高脂转普通膳食组(HTN组)、高脂膳食+有氧运动组(EH组),各10只,继续喂养8周后,测定血清中的甘油三酯(TG)和胆固醇(CHO),内脏脂肪和肝脏中Visfatin mRNA的水平及血清中总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-px)、丙二醛(MDA)及Visfatin的含量。结果:(1)HD组的TG、CHO及血清Visfatin、肝脏Visfatin mRNA水平均高于ND组,内脏脂肪Visfatin mRNA水平较ND组降低;(2)HTN组的内脏脂肪和肝脏Visfatin mRNA水平较OB组下降;EH组血清Visfatin和肝脏Visfatin mRNA水平较OB组下降,内脏脂肪Visfatin mRNA水平升高;(3)EH组血清T-SOD及GSH-px水平上升,MDA水平下降;(4)血清SOD和Visfatin水平有显著的相关性且呈正相关(r值分别为0.505、0.627、0.520,P<0.05)。结论:有氧运动和饮食干预可降低肥胖机体血清的Visfatin及肝脏Visfatin mRNA水平,降低肥胖机体的OS水平,并增强机体抗氧化能力,从而减少了肥胖对机体的损伤。
Objective To study the relationship between oxidative stress （OS）and serum Visfatin levels of obese mice during intervention of aerobic exercise and diet control. Methods 40 C57BL/6J male mice were divided into the control group （ND, n = 10）and high-fat diet group （HD,n = 30）. Following development of obesity,mice in HD group were again randomly and equally divided into the followingthree groups：control group （OB）,normal diet group （HTN）and exercise plus high-fat diet group（EH）. After eight-week experiment, the levels of TG, CHO ,T-SOD, GSH-px, MDA, serum Visfatin and Visfatin mRNA levels in visceral adipose tissue （VAT）and liver were detected. Results （1）Comparing with group ND,the levels of TG,CHO,serum Visfatin and Visfatin mRNA level in liver in group HD increased; while Visfatin mRNA level in VAT decreased. （2）Comparing with group OB, the levels of Visfatin mRNA in VAT and liver in group HTN decreased; and level of serum Visfatin and Visfatin mRNA level in liver in group EH decreased,while level of Visfatin mRNA in group VAT increased. （3）The levels of OS and GSH-px were higher and the level of MDA in group EH were lower than that in group OB. （4）The level of serum Visfatin was positively correlated with the level of SOD（r = 0.505 ,P 〈 0.05）,GSH-px（r = 0.627,P 〈 0.01）and MDA （r = 0.520,P 〈 0.05）. Conclusion Aerobic exercise and diet control down-regulate the levels of OS, serum Visfatin, and Visfatin mRNA level, and improve antioxidant capacity of obese mice.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2014年第1期52-57,共6页
Chinese Journal of Sports Medicine
基金
2012年国家大学生创新创业训练计划(201210043096)
