摘要
以橄榄油为唯一碳源,采用平板变色圈法,从土壤中筛选到1株脂肪酶高产菌XYU-6,经生理生化试验鉴定及16S rDNA序列分析,XYU-6被鉴定为洋葱假单胞菌(Burkholderia cepacia).设计单因素试验,对其产酶条件进行优化,优化后的培养条件为:葡萄糖1.00%,蛋白胨2.00%,豆油1.00%,KH2PO40.35%,K2HPO40.15%,MgSO40.05%,初始pH值为7.0,培养温度30℃,培养48 h,酶活可达24.1 U·mL-1,是优化前的1.65倍.
A lipase producing strain XYU-6 was isolated from the soil by the method of flat discolor circle with olive oil as sole carbon source. XYU-6 was identified as Burkholderia cepacia by 16S rDNA sequencing and its biochemi- cal and physiological characteristics. The optimal conditions, glucose 1. 00%, peptone 2. 00%, soya-bean oil 1.00% ,KH2PO4 0.35% ,K2HPO4 0.15% and MgSO4 0.05% were obtained through one-factor experimental de- sign. Furthermore, the highest lipase activity 24.1 U mL-lwas achieved under the optimum conditions with initial pH 7.0 ,culture temperature 30 ℃ and culture time 48 h. These results indicated that the lipase activity was im- proved 1.65 fold compared to the original conditions.
出处
《江西师范大学学报（自然科学版）》
CAS
北大核心
2014年第1期14-18,共5页
Journal of Jiangxi Normal University(Natural Science Edition)
基金
江西省青年科学基金(20122BAB214011)资助项目
关键词
脂肪酶
洋葱假单胞菌
筛选
鉴定
优化
lipase
Burkholderia cepacia
screening
identification
optimization
