摘要
目的观察癌痛消药物血清对人肝癌细胞SMMC-7721和Bel-7404体外增殖及凋亡的影响,探讨其作用机制。方法癌痛消饱和溶液按86.4、43.2、21.6 g/kg剂量给予SD大鼠灌胃1周,取血制备含药血清,加入SMMC-7721及Bel-7404肝癌细胞培养液。不同浓度癌痛消药物血清处理人肝癌细胞SMMC-7721和Bel-7404肝癌细胞后,MTT法检测细胞增殖变化,流式细胞术检测细胞凋亡。结果体外研究显示,癌痛消方药物血清低、中、高剂量组对人肝癌细胞SMMC-7721和Bel-7404均具有不同程度的抑制作用,且这种抑制作用在一定范围内呈浓度依赖性,癌痛消药物血清对人肝癌细胞SMMC-7721和Bel-7404有促凋亡作用。结论癌痛消方药物血清在体外对人肝癌细胞SMMC-7721和Bel-7404肝癌细胞有抑制增殖作用和促凋亡作用。
Objective To observe the anti-cancer effect in vitro of Aitongxiao Granules containing serum on SMMC-7721 and Bel-7404 liver cancer cells, thus revealing the anti-cancer mechanism of the treatment in adjusting proliferation and apoptosis. Methods Male Sprague-Dawley rats were administered by gastrogavage Aitongxiao decoction of 86.4, 43.2, 21.6 g/kg twice a day for 1 week. Using serum pharmacologic method, the proliferation of SMMC-7721 and Bel-7404 liver cancer cells were determined by MTT chromatometry after co-cultured with medicated serum containing different concentration of Aitongxiao decoction. The apoptosis of SMMC-7721 and Bel-7404 liver cancer cells were detected by flow cytometry. Results The research in vitro showed that Aitongxiao Granules containing serum low, medium and high dose groups had varying degrees of inhibition on hepatoma cells, and this inhibition showed a concentration dependence within a certain range. Aitongxiao Granules containing serum could induce apoptosis of human hepatoma SMMC-7721 cells and Bel-7404 cells. Conclusion Aitongxiao Granules showed effect of inhibiting proliferation and inducing apoptosis on SMMC-7721 and Bel-7404 cells in vitro.
出处
《中国中医药信息杂志》
CAS
CSCD
2014年第3期58-61,共4页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(81160438)
广西中医药大学第一附属医院青年基金(GZYKJ2011017)
关键词
癌痛消
药物血清
肝癌
增殖
凋亡
大鼠
Aitongxiao Granules
drug containing serum
hepatoma
proliferation
apoptosis
rats
