摘要
利用比较灵芝液态深层发酵菌丝体含量的方法,选择G0119、G0130和G0154作为亲本菌株。通过原生质体单核化技术,获得了108株灵芝单核菌株。以单核菌株作为杂交育种材料,将筛选出的单核菌株进行两两配对,共得到63株杂交菌株,通过拮抗反应验证,证实63株杂交菌株是真正的杂交菌株。其次,用Logistic模型对灵芝杂交菌株菌丝体生长、葡萄糖消耗和总三萜合成的比速率进行了计算与分析。结果表明,杂29和杂62灵芝杂交菌株菌丝体的最大比生长速率分别为0.147 h-1和0.154 h-1,葡萄糖的最大比消耗速率分别为0.011h-1和0.042 h-1,三萜的最大比合成速率分别为0.0010 h-1和0.001 9 h-1。因此,利用原生质体单核化方法制备的杂交菌株不同于亲本菌株,其动力学参数分析表明,杂交菌株对底物的利用和三萜产物的合成与亲本菌体比较有较大的提高。
In this study, the G0119, G0130 and G0154 strains were screened out as the hybrid parent according to their mycelium concentration of liquid submerged fermentation. 108 mononuclear strains were obtained by protoplast monokaryogenesis method. Mononuclear strains were selected as cross-breeding materials. Two monokarryotic strains of each parental strain with different genotype were mated, and 63 hybrids were obtained, which were identified by antagonist response. Moreover, the unstructured kinetics of Ganoderma lucidum hybrid strain liquid submerged fermentation process was investigated using Logistic mode, and the specific rates of cell growth, glucose consumption and triterpenes formation were analyzed and calculated. The results showed that the maximum specific rate of cell growth was 0. 147 h-1 and 0. 154 h- 1, the maximum specific rate of glucose consumption was 0.011 h- 1 and 0. 042 h-1 and the maximum specific rate of triterpenes formation was 0. 0010 h-1 and 0. 0019 h-1 for hybrid 29 and hybrid 62. Hence, the hybrid from protoplast monokaryogenesis method was different from parents. Its kinetic parameters showed that glucose consumption and triterpenes formation were improved compared with parents.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第3期107-112,共6页
Food and Fermentation Industries
基金
上海市科技兴农重点攻关项目“食用菌重要功能基因的发掘和新品种培育”(沪农科攻字(2011)第1-2号)
国家十二五科技支撑课题:珍稀真菌高值利用技术研究与产品开发(2012BAD36B05)
关键词
灵芝
杂交育种
菌丝体
深层发酵
动力学
Ganoderma lucidum, hybrid, mycelium, submerged fermentation, kinetics
