摘要
建立了免疫亲和柱净化—超高效液相色谱法快速测定粮食中玉米赤霉烯酮（Zearalenone,ZEN）的方法.ZEN的保留体积为0.47 mL,检出限为5 pg,在10～500.0 pg范围内呈线性相关,相关系数R2值为0.999 9;在小麦、玉米样品中的加标回收率分别为85％～ 102％和83％～ 112％,精密度为2.7％～6.9％.本方法从样品前处理到分析整个过程小于45 min,简便快速、灵敏度高、重现性好、溶剂用量少,适用于粮食中玉米赤霉烯酮的快速测定.
A rapid method, immunoaffinity column - ultra - high performance liquid chromatography, was established for determining zearalenone （ZEN） in grain. The retention volume of ZEN was 0.47 mL, the detection limit 5 pg. The linear detection ranges of ZEN was 10 -500 pg with correlation coefficients （R2） of 0. 999 9. Recovery rates in grains （wheat, corn） spiked with ZEN was in the range of 85% - 102%, 83% -112%, respectively, with the relative standard deviation of 2.7% -6.9%. The method with analytical time less than 45 min was suitable for the determination of ZEN in the raw grain, with the advantages of simplicity, rapidness, sensitivity, good reproducibility, and less solvent.
出处
《粮油食品科技》
北大核心
2014年第2期71-75,共5页
Science and Technology of Cereals,Oils and Foods
基金
国家科技部863课题(2012AA101609)
关键词
粮食
玉米赤霉烯酮
超高效液相色谱
免疫亲和柱
grain
zearalenone （ZEN）
ultra - high performance liquid chromatography （ UPLC ）
im-munoaffinity column （ IAC ）
