摘要
目的:在对小麦低聚肽的基础理化成分和分子量分布进行分析的基础上,观察不同浓度的小麦低聚肽对人肝癌细胞株HepG2胆固醇代谢的影响。方法:将培养的HepG2细胞随机分为5组:对照组、2、4、6、8g/L小麦低聚肽实验组。经小麦低聚肽作用24h后,进行反转录-聚合酶链反应(RT-PCR),定量分析低密度脂蛋白受体(LDLR)和3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA的表达。结果:与对照组相比,经小麦低聚肽作用后,各实验组HepG2细胞LDLR mRNA表达明显增加(p<0.05或p<0.01),其中6g/L小麦低聚肽组的LDLR mRNA表达增加最多;HMGCR mRNA表达均降低,2、4、6g/L小麦低聚肽组的HMGCR mRNA表达极显著降低(均为p<0.01),其中2g/L小麦低聚肽组的HMGCR mRNA表达降低最多。结论:不同浓度的小麦低聚肽均能升高HepG2细胞内LDLR mRNA水平和降低HMGCR mRNA水平,从而起到降低胆固醇的作用,为其开发利用提供了一定的实验依据和理论基础。
Objective： To observe the influence of wheat oligopeptides （WOP）on cholesterol metabolism-related genes in HepG2 ceils on the basis of compositions and molecular weight distribution of WOP.Method. HepG2 cells were divided into five groups in random .the control ,2,4,6 and 8g/L of WOP groups.After treated by WOP for 24h, RT-PCR was used to test the changes of expression of low density lipoprotein receptor（LDLR） and 3-hydroxy-3- methylglutaryl coenzyme A reductase （HMGCR）genes. Result, Compared with the control group, after treated by WOP,LDLR mRNA level was obviously increased in all test groups（p 〈0.05,p 〈0.01 ） ,with LDLR mRNA level in 6g/L of WOP group increased the most.HMGCR mRNA level was all decreased in all test groups.The expression of HMGCR mRNA was extremely significantly decreased in 2,4 and 6g/L groups ,with HMGCR mRNA level in 2g/L of WOP group decreased the most.Conclusion =WOP could increase LDLR mRNA level and decrease HMGCR mRNA level at all the test concentrations,therefore WOP could reduce the levels of cholesterol in HepG2 cells.The results of this paper provide experiment and theoretical basis for the development and utilization of WOP.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第7期126-129,共4页
Science and Technology of Food Industry
基金
国家十二五科技支撑项目(2012BAD33B04-02)
国家高技术研究发展计划(863计划)项目(2013AA102205-02)
北京市科委科技创新基地培育与发展工程项目(Z121106002812040)
科技北京百名领军人才培养工程项目(Z131110000513026)
