摘要
为探讨脂多糖(LPS)对母鸡产蛋及产道先天免疫和应激相关基因表达的影响,试验采用40羽46周龄温氏土种母鸡,随机分为2组,对照组腹腔注射1 mL·kg-1生理盐水,试验组腹腔注射1 mg·kg-1的LPS(1 mg·mL-1)。记录注射后12 d的采食量和产蛋率。待2组母鸡生产性能恢复正常后重复LPS注射,24 h后处死母鸡,采集血液、输卵管峡部和子宫,检测血液生化指标、输卵管峡部和子宫氧化应激指标(总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和丙二醛(MDA)),并测定峡部和子宫免疫、增殖、凋亡和应激相关基因的mRNA水平。结果表明:LPS组母鸡采食量和产蛋率显著降低(P<0.05);血清白蛋白、球蛋白和全蛋白浓度显著降低(P<0.05);母鸡峡部细胞因子IL-1βmRNA水平显著升高(P<0.05);子宫增殖细胞核抗原(PCNA)的mRNA水平显著降低(P<0.05)。结论:LPS急性刺激显著降低了母鸡采食量和产蛋率,这与血液免疫球蛋白含量下降,峡部炎症因子表达上调和子宫增殖相关基因表达下调有关。
To investigate the effect of lipopolysaeeharide （LPS） on egg production and oviduct innate immunity- and stress-related gene expression of hens, 46-week-old Wen＇s hens for 40 were divided into two groups. The hens of the control group were intraperitoneally injected of 1 mL. kg-1 saline, and the hens of the LPS group were intraperitoneally injected with l mg. kg-1 LPS. Feed intake and egg production of hens were recorded for 12 days after the injection. When the laying performace returned to normal, hens were injected with LPS again and humanely killed to collect bloods, isthmus and uterus 24 h after LPS injection. Blood parameters,oxidative stress indices （ T-AOC, SOD and MDA） of isthmus and uterus, and expression of proliferation, apoptosis, immunity and stress-related genes in isthmus and uterus were measured. The results showed that, LPS injection dramatically reduced feed intake and egg production（ P〈0.05 ）, which was associated with greatly decreased plamsa albumin, globulin and holoprotein concentrations（ P〈0.05 ）. Real-time PCR revealed that IL-1β expression （P〈0.05）in isthmus was significantly up-regulated, while proliferating cell nuclearantigen（ PCNA,P〈0.05 ）in uterus was reduced 24 h after LPS injection. In conclusion, LPS reduces feed intake and egg production in hens,which is associated with reduced immunoglobulin in the blood,increased inflammation in isthmus, and decreased cell proliferation in uterus of hens.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2014年第2期92-98,共7页
Journal of Nanjing Agricultural University
基金
国家公益性行业(农业)科研专项(201003011)
江苏高校优势学科建设工程资助项目
关键词
脂多糖
产蛋
免疫
应激
母鸡
lipopolysaccharide
egg production
innate immune
stress
hens
