摘要
目的了解常用项目在不同生化检测系统上的试剂空白测定值情况,探讨用稀释法检测丙氨酸氨基转移酶(ALT)时,高空白测定值对其结果的影响及纠正办法。方法在迈瑞BS-820、西门子Dimension Rxl Max全自动生化系统上共观察了ALT、三酰甘油(TG)、碱性磷酸酶(ALP)、转肽酶(GGT)、乳酸脱氢酶(LDH)、磷(Phos)、钙(Ca)7种生化项目的空白测定值。在Dimension Rxl Max生化系统上,对3份血清标本分别按1∶2、1∶4及1∶8稀释后再测ALT,所得结果乘以稀释倍数,并进行了纠正。结果在迈瑞BS-820生化系统上,ALT、TG、ALP、GGT、LDH、Phos、Ca 7种项目的空白测定值分别是:1.0 U/L、0.0 mmol/L、3.0 U/L、1.0 U/L、-1.0 U/L、0.0 mmol/L、0.0 mmol/L;在西门子Dimension Rxl Max系统上,依次为18.0 U/L、0.0 mmol/L、10.0 U/L、2.0 U/L、0.0 U/L、0.0 mmol/L、0.0 mmol/L。在Dimension Rxl Max系统上,3份血清标本经系列稀释测得的ALT结果乘以稀释倍数后,分别是64、104、176 U/L;108、144、224 U/L;246、292、360 U/L。经纠正后,则为46、50、50 U/L;90、90、98 U/L;228、238、234 U/L。结论在不同的系统上,这些生化项目的空白测定值会存在差异,应加以重视。在Dimension Rxl Max生化系统上,由于高空白测定值的存在,血清标本经稀释后再测ALT时,结果仅乘以稀释倍数是不准确的。经合理校准后的结果才是可靠的。
Objective To investigate the measured values of some test items＇ reagent blanks in different biochemical detection systems; and to explore the effect of high measured value of alanine aminotransferase （ALT） reagent blank on the detected results of ALT when serum samples were detected after being diluted,as well as the corrective methods.Methods The blank measurement values of seven different test items were observed in both Mindray BS-820 and Siemens Dimension Rxl Max automatic biochemical systems,including ALT,triacylglycerol （TG）,alkaline phosphatase （ALP）,γ-glutamyl transferase （GGT）,lactate dehydrogenase （LDH）,phosphorus （Phos） and calcium （Ca）.In Dimension Rxl Max system,the ALT activities in three serum samples were detected after being diluted by 1∶2,1∶4,1∶8.The results were multiplied by the dilution factors,and corrected.Results The blank measurement values of ALT,TG,ALP,GGT,LDH,Phos and Ca were 1.0 U/L,0.0 mmol/L,3.0 U/L,1.0 U/L,-1.0 U/L,0.0 mmol/L,0.0 mmol/L in Mindray BS-820 system; and they were 18.0 U/L,0.0 mmol/L,10.0 U/L,2.0 U/L,0.0 U/L,0.0 mmol/L,0.0 mmol/L in Dimension Rxl Max system.In Dimension Rxl Max system,the ALT results in three samples after being multiplied by the dilution factors were 64,104,176 U/L; 108,144,224 U/L; 246,292,360 U/L.However,they were 46,50,50 U/L; 90,90,98 U/L; 228,238,234 U/L after being corrected.Conclusion The measured values of these reagent blanks are various in different biochemical detection systems,and we should pay more attention to it.The measurement results of ALT must be corrected when serum specimens are detected in Siemens Dimension Rxl Max system after being diluted,because of the existence of high measured value of ALT reagent blank.
出处
《中国医药导报》
CAS
2014年第6期149-151,共3页
China Medical Herald
关键词
丙氨酸氨基转移酶
空白测定值
稀释
影响
纠正
Alanine aminotransferase
Measured value of reagent blank
Dilution
Effect
Correction
