摘要
目的为了消除夏荔芪胶囊在微生物限度检查中药用成分的抑菌作用,建立其适宜的微生物限度检查方法。方法本试验采用平皿法和培养基稀释法对该品种进行微生物限度检查方法验证,通过三次独立平行试验,各试验菌的回收率均可达到70%以上。结论采用平皿法、培养基稀释法联用检查细菌数,霉菌及酵母菌数,可客观地反映该药物中微生物的污染状况,且可操作性强。
Objeetive To develop an appropriate protocol to remove the antirnicrobial activity of Xialiqi Capsule and establish a suitable method for the microbial enumeration testing. Methods A combination of the plating procedure and media dilution method was used to do the validation. In the three independent parallel tests, the mean counts of each of the test organisms were all not less than 70 %. Colusion The combination of the plating procedure and media dilution method was verified to be an appropriate method for microbial enumeration testing. The validated method is easily operational, and can reflect the microbial contamination condition objectively.
出处
《中国中医药现代远程教育》
2014年第1期154-155,共2页
Chinese Medicine Modern Distance Education of China
关键词
夏荔芪胶囊
微生物限度检查
平皿法
培养基稀释法
回收率
Xiatiqi Capsule
Microbial limit test
Plating procedure
Media dilution method
Recovery rate
