摘要
为明确人参中咪鲜胺的残留量及环境安全性，确保人参产品质量安全，采用高效液相色谱-串联质谱法测定25%咪鲜胺乳油在人参根、茎、叶及土壤中的残留动态及最终残留量。样品经乙腈提取，PSA固相萃取柱净化后以液相色谱分离，采用电喷雾电离源，以质谱正离子扫描多反应监测模式进行定量分析，外标法定量。结果表明，咪鲜胺在人参根、茎、叶及土壤中的回收率为86.8%-90.8%，相对标准偏差为3.95%-7.15%。施药剂量为1000 g·hm-2（以有效成分计）时，咪鲜胺在人参根、茎、叶和土壤中的降解半衰期为8.39-13.20 d。施药剂量为500-1000 g·hm-2时，施药后60 d咪鲜胺在人参根、茎、叶和土壤中的最终残留量均≤0.0417 mg·kg-1，未超过日本（0.05 mg·kg-1）、欧盟（0.20 mg·kg-1）、韩国（0.30 mg·kg-1）规定的最大残留限量值，该药按推荐剂量使用安全。
To determine the residual dynamics and environmental safety of prochloraz 25% EC and ensure the quality safety of ginseng products, a high performance liquid chromatography coupled with electrospray mass spectrometry （HPLC-MS/MS） method was developed for determining residual dynamics and final residues of prochloraz in ginseng root, stem, leaf and soil. The samples were extracted with acetonitrile, cleaned-up with PSA solid phase extraction cartridge, separated by liquid chromatography and analyzed with the multiple reaction monitoring （MRM） in positive ion mode by employing the external standard method, using electron spray ionization source （ESI）. The results show-ed that the recoveries of prochloraz were 86.8%-90.8%, and the relative standard deviations were 3.95%-7.15%in ginseng root, stem, leaf and soil. The half-lives of prochloraz were from 8.39 to 13.20 d at spraying dosage of 1 000 g·hm-2. Final residues of prochloraz were less 0.0417 mg·kg-1 in ginseng root, stem, leaf and soil, below the MRLs of Japan （0.05 mg·kg-1）, EU （0.20 mg·kg-1） and Korea （0.30 mg·kg-1） over 60 d, which at spraying dosage range from 500 to 1 000 g·hm-2. Therefore, it is safe to apply prochloraz to ginseng at the recommended dosage.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2014年第3期25-30,共6页
Journal of Northeast Agricultural University
基金
吉林省世行贷款农产品质量安全项目(2011-Z24)
吉林省人参产业发展专项资金项目(2011-12)
