摘要
目的：探讨鸦胆子油注射液在体内外对 H22细胞增殖的抑制作用及其作用机制。方法取 H22细胞培养，采用 MTT 法检测鸦胆子油对细胞增殖的影响；应用 H22细胞建立小鼠荷瘤模型，给予鸦胆子油治疗，计算抑瘤率、脾脏指数和胸腺指数，采用 MTT 法检测鸦胆子油对荷瘤小鼠脾细胞增殖的影响，采用双抗体夹心ABC-ELISA 法检测荷瘤鼠血清 TNF-α水平，采用放射免疫分析法检测转化生长因子（TGF）-α水平。结果鸦胆子油在（10~160）μg/ml 浓度范围内对 H22细胞的增殖抑制率为34.1%~52.31%，与对照组比有统计学意义；给予鸦胆子油25和50 mg＆#183;kg-1灌胃后，荷瘤小鼠瘤质量分别为（1.095±0.301） g 和（0.920±0.250） g，血清 TGF-α分别为（11.172±0.639） pg/ml 和（14.438±0.587） pg/ml，均较未治疗荷瘤动物显著下降[分别为（1.867±0.554） g 和（16.354±0.762） pg/ml]，TNF-α水平分别为（28.132±2.456） pg/ml 和（26.521±3.267） pg/ml，较未治疗荷瘤动物显著升高[（20.231±2.614） pg/ml，P〈0.05]；与对照组比，无论是 Con A 还是 LPS 刺激，荷瘤动物脾细胞增殖受到抑制（P〈0.05）。与荷瘤动物比，鸦胆子油体内给药则可促进脾细胞增殖，但对脾脏指数和胸腺指数无明显影响。结论鸦胆子油体内外均能抑制肝癌 H22细胞的生长，其抗肿瘤作用可能与改善荷瘤小鼠的脾细胞功能有关。
Objective To investigate the inhibitory effect and related mechanisms of Brucea oil on prolifer-ation of H22 hepatoma cell line. Methods H22 cells cultured in vitro were incubated with different concentra-tion of Breucea oil （10~160 μg/ml） and cell proliferation was determined by MTT assay;Mice bearing H22 tu-mors were treated with Breacea oil for 10 days,and then the mice were scarified and tumor inhibitory rate, spleen index and thymus index were calculated;MTT was performed to detect the proliferation of spleen cells from tumor-bearing mice;The serum TNF-α was measured by enzyme-linked immunosorbent assay and serum trans-forming growth factor-α （TGF-α） levels by radioimmunoassay. Result The cell proliferation was inhibited by Brucea oil to 34.1%~52.31% of that of in the control at concentrations of 10 to 160 μg/ml;At concentration of 25 and 50 mg＆#183;kg-1,the tumor mass in tumor-bearing mice were reduced to （1.095±0.301） g and （0.920±0.250） g,respectively,and serum TGF-α levels decreased to（11.172±0.639） pg/ml and （14.438±0.587） pg/ml,respective-ly,significantly lower than those in non-treated tumor-bearing mice[（1.867±0.554） g and （16.354±0.762） pg/ml, respectively];In contrast,serum TNF-α levels increased to（28.132±2.456） pg/ml and（26.521±3.267）pg/ml,respec-tively,significantly higher than that in non-treated tumor-bearing mice [（20.231±2.614） pg/ml,P〈0.05];Brucea oil also promoted the proliferation of spleen cells,but had no significant effect on the spleen index and thymus index as compared to those in tumor-bearing mice. Conclusion Brucea oil inhibits the proliferation of H22 cells in vitro and in vivo,and the anti-tumor effect might be related to the regulation of spleen cells in tumor-bearing mice.
出处
《实用肝脏病杂志》
CAS
2014年第2期180-183,共4页
Journal of Practical Hepatology
关键词
H22
细胞
鸦胆子油
脾细胞
肿瘤坏死因子-Α
转化生长因子-Α
H22 cell line
Brucea oil
Proliferation
Spleen cells
Tumor necrosis factor -α
Transforming growth factor-α
