摘要
目的:建立益肝防石颗粒中马钱苷的含量测定方法。方法:采用高效液相色谱法对马钱苷进行了含量测定;色谱柱为Diamonsil-C_(18)柱(250 mm×4.6 mm,5μm);流动相为乙腈-水(10:90);流速为1.0 ml·min^(-1);检测波长为240 nm。结果:HPLC法定量分离效果好,马钱苷进样量在22.5~720.0μg·ml^(-1)范围内呈良好的线性关系(r=0.999 8),平均回收率为97.35%,RSD为1.24%(n=6)。结论:该方法简单、准确、重复性良好,可用于益肝防石颗粒中马钱苷的含量测定。
Objective： To establish a method to determine loganin in Yigan Fangshi granules. Methods： HPLC was used to deter- mine loganin on a Diamonsil-C18 column（250 mm ×4.6 mm, 5 μm） using acetonitrile-water（ 10： 90） as the mobile phase. The flow rate was 1.0 ml · min- 1 and the detection wavelength was at 240 nm. Results ： There was a good linearity within the range of 22.5- 720.0 μg · ml - 1 （ r = 0.999 8） for loganin. The average recovery was 97.35 % with RSD of 1.24% （ n = 6）. Conclusion： The meth- od is simple,accurate and reproducible, which can be used in the determination of loganin in Yigan Fangshi granules.
出处
《中国药师》
CAS
2014年第3期514-515,共2页
China Pharmacist
基金
军队医疗机构制剂标准提高科研专项课题(编号:13ZJZ02)
