摘要
目的观察Long Evans大鼠视神经损伤后不同时间基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)及其受体CXCR4在视网膜中的分布及表达变化。方法将21只成年Long Evans大鼠分为正常组和视神经损伤后1、3、5、7、15 d及30 d组,每组3只,采用免疫荧光染色和免疫印迹法(Western blot)检测各组大鼠视网膜SDF-1、CXCR4的分布及表达变化。结果免疫荧光染色发现视神经钳夹伤大鼠SDF-1主要定位表达于视网膜内、外界膜之间的星形胶质细胞及Müller细胞,并与GFAP共表达,SDF-1阳性细胞数量随视神经损伤时间延长先增多后减少,伤后5 d达高峰。CXCR4主要表达于视网膜神经节细胞层、外核层及内核层;Western blot证实视网膜SDF-1的水平随视神经损伤出现先升高后降低的趋势,与正常组相比,视神经损伤组第1、3、5、7天水平显著升高(P<0.05)。而CXCR4的表达在视神经损伤后各时间点无明显改变(P>0.05)。结论视神经损伤后,视网膜星形胶质细胞及Müller细胞中的SDF-1表达先增强后减弱,且与视神经损伤后胶质活化相关。
Objective To observe the distributions factor-1 （SDF-1） and its receptor CXCR4 in the retinas during and expression profiles of stromal cell-derived repair period after partial optic nerve injury in Long Evans rats. Methods A total of 21 adult rats were randomly and equally divided into 7 groups, normal group, the injury group, and the groups in 1, 3, 5, 7, 15 and 30 d after optic nerve crushing. Immunofluores eence assay and Western blotting was applied to detect the location and the quantity of SDF-1 and CXCR4 expression in the retina. Results Immunofluoreseence staining revealed that the expression of SDF-1 was co localized in the activated GFAP-positive astrocytes and MUller cells between the pre-retinal and post-retinal membrane. The amount of SDF-1 positive cells were in a trend of increasing to decreasing with the injury time prolonging, and reached a summit in 5 d after injury. CXCR4 staining was mainly present in the ganglion cell layer, inner nuclear layer and outer nuclear layer of the retina. Western blotting revealed that the expression of SDF-1 protein was increased at the first day and then decreased at the 7th day after injury. The expression of SDF-1 in 1, 3, 5 and 7 d after optic nerve injuring was increased significantly than normal group （P 〈 O. 05 ）. While, the expression of CXCR4 protein had no change after injury （P 〉 0.05 ）. Conclusion The quantity of SDF-1 is increased first and then decreased in the astrocytes and Mtiller cells after optic nerve injuring, which is related to the activation of glial cells after injury.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2014年第7期645-649,共5页
Journal of Third Military Medical University
基金
国家自然科学基金(30901645
81270996)~~
