摘要
目的:观察红花黄色素(SY)抑制血小板激活因子(PAF)诱发的内皮细胞炎性因子蛋白表达升高及抑制PAF与其受体特异性结合的作用。方法:用酶联免疫吸附测定法(ELISA)检测SY缓解1μmol/L PAF刺激脐静脉内皮细胞株Eahy926培养液中的炎症因子白细胞介素(IL)-1β、IL-6和肿瘤坏死因子(TNF)-α的蛋白表达水平的变化;以放射配基结合试验观察SY抑制[3H]标记的PAF与家兔血小板膜和膜蛋白PAF受体特异性结合的作用。结果:与正常组比较,PAF损伤组内皮细胞培养上清液中IL-1β、IL-6及TNF-α蛋白表达水平明显升高(P<0.001),SY处理组(浓度为4.5×10-5,4.5×10-4,4.5×10-3g/L)时可明显抑制Eahy926培养液中IL-1β、IL-6及TNF-α蛋白表达水平的升高(P<0.05,0.01,0.001);受体结合实验结果表明浓度为16.49g/L的SY可抑制0.17 nmol/L[3H]PAF与家兔血小板膜的特异性结合,浓度为11.17和22.08g/L的SY分别可抑制0.083及0.25nmol/L[3H]PAF与兔血小板膜蛋白的特异性结合。结论:SY通过抑制PAF与其受体的特异性结合缓解了PAF诱发的血管内皮细胞炎症介质IL-1β、IL-6及TNF-α蛋白的高表达。
Objective：To observe the inhibitory effect of samor yellow （SY） on endothelium inflammatory factor proteins expression elevation triggered by platelet activating factor（PAF）.Methods：Umbilicus vein endothelium cell line Eahy926 was used.The cell culture supernatants were collected from Eahy926 cells 4 h after SY and PAF addition.The concentrations of IL-1β,IL-6 and TNF-o were measured by ELISA technology.The specific binding of [3H] PAF to rabbit platelet membrane and platelet membrane protein were observed by radio ligand binding assay （RLBA）.Results：Endothelium IL-1β,IL-6,TNF-α protein expression levels increased after PAF addition and SY can attenuate these changes.The PAF receptor specific binding inhibition effects of SY were observed.Conclusion：The specific binding of PAF to its receptor can be antagonized by SY and SY is effective to inhibit endothelium IL-1β,IL-6,and TNF-α protein expression elevation induced by PAF.
出处
《心肺血管病杂志》
CAS
2014年第2期281-285,共5页
Journal of Cardiovascular and Pulmonary Diseases
基金
国家自然科学基金科学基金资助项目(81270103)
关键词
红花黄色素
血小板激活因子
受体结合试验
内皮细胞
炎症介质
Safflor yellow
Platelet activating factor
Receptor binding assay
Inflammatory factor
Endothelium
