摘要
目的了解山东济南地区急性上呼吸道感染病毒病原谱的流行及构成,为预防策略制定、临床诊断、治疗提供病原学依据。方法对2010年9月—2011年8月在山东大学齐鲁儿童医院采集的上呼吸道标本分别用荧光PCR方法检测博卡病毒,多重PCR方法检测12种病毒,阳性标本进行流感病毒、腺病毒和呼吸道合胞病毒的分离和鉴定。结果 174份样本中检出阳性结果 84份,阳性率为48.28%,在整个病毒感染中,检出阳性率最高的为腺病毒(20.43%),其次为甲型流感病毒(FluAV)(18.28%)、副流感病毒3(HPIV-3)(15.05%)、甲型呼吸道合胞病毒(RSVA)(11.83%)、冠状病毒(HCoV-229E/NL)63(10.75%)、乙型流感病毒(FLUBV)(6.45%)、HCoVOC43/HKU1(5.38%)和乙型呼吸道合肥病毒(RSVB)(4.30%);在混合感染中,检出阳性率较高的为HAdV(22.22%)、RSVA(22.22%)和FluAV(16.67%);<5岁主要以感染A型流感病毒和A型呼吸道合胞病毒为主,≥5岁主要以感染腺病毒为主;在检出的阳性标本中,共分离到12株流感病毒和9株腺病毒。结论济南地区上呼吸道感染病例的主要病毒为腺病毒、FluAV、HPIV-3、RSAV、HCov-229E/NL63和FluBV,各种呼吸道病毒随年龄不同、流行季节中月份不同。
Objective To study the prevalence and viral etiology of acute upper respiratory infection among children in Ji＇nan city,Shandong province and to provide reference for making preventive strategy,clinical diagnosis and treatment.Methods From September 2010 to August 2011,we collected the samples of throat swab from the acute upper respiratory infection patients in Qilu Children Hospital of Shandong University.The samples were detected with real-time PCR for human bocavirus（HBoV）and multiplex PCR for 12 kinds of respiratory virus,respectively.Madin-Dardy canine kidney（MDCK）cells and Hep-2 cells were used to isolate influenza virus（FLU）,human adenovirus（HAdV）and respiratory syncytial virus（RSV）.Results Totally 84 positive samples were detected from the 174 samples,with a positive rate of 48.28%.HAdV（20.43%）was the most common virus,followed by Flu A（18.28%）,human parainfluenza viruses -3（HPIV-3）（15.05%）,RSVA（11.83%）,human coronavirus 229E/NL63（HCoV-229E/NL63）（10.75%）,Flu B（6.45%）,human coronavirus OC43/HKU1（HCoV-OC43/HKU1）（5.38%）,and RSVB（4.30%）.For the co-infections,HAdV（22.22%）and RSVA（22.22%.2.）were strains mostly detected,followed by Flu A（16.67%）.The children under 5 years were more likely to have Flu A and RSVA infection and persons ≥5 years old were more likely to have 12 HAdV influenza viruses and 9 HAdV strains.Conclusion This study underlines the important of viral pathogens in acute upper respiratory infection in Ji＇nan city,Shandong province.The major viral pathogens are HAdV,Flu A,HPIV-3,RSVA,HCoV-229E/NL63 and Flu B with different seasonal and special distributions.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2014年第4期520-523,共4页
Chinese Journal of Public Health
基金
国家"十一.五"科学技术重大专项(2009ZX10004-202)
山东省科技厅计划项目(2009GG10002054)
关键词
急性呼吸道感染
病原学
多重RT-PCR
acute upper respiratory infection viral etiology multiplex RT-PCR
