摘要
目的:研究何首乌提取物对正常与荷肝癌小鼠的急性肝毒性。方法:50只ICR正常小鼠随机均分为正常对照(等容0.5%CMC-Na溶液)组、何首乌总提物(1 500 mg/kg)组、何首乌乙醇提取物大孔树脂吸附后50%乙醇洗脱部位(简称R50部位,500 mg/kg)组、2,3,5,4’-四羟基二苯乙烯-2-O-β-d葡糖苷(TSG,185 mg/kg)组、大黄素-8-O-β-d-葡糖苷(EG,2 mg/kg)组,灌胃给药,每天1次,连续10 d。40只荷肝癌小鼠随机均分为模型(等容0.5%CMC-Na溶液)组与R50部位高、中、低剂量(1 000、500、250 mg/kg)组,另设正常对照(等容0.5%CMC-Na溶液)组,灌胃给药,每天1次,连续10 d。观察小鼠一般状况,测定小鼠血液丙氨酸氨基转移酶(ALT)与天冬氨酸氨基转移酶(AST)活性,称定小鼠肝质量、体质量,计算肝脏系数。结果:与正常对照组比较,何首乌总提物组、R50部位组、TSG组、EG组小鼠一般情况、ALT和AST活性、肝脏系数无明显改变(P>0.05)。与正常对照组比较,模型组小鼠AST和ALT活性增强、肝脏系数升高,差异有统计学意义(P<0.05);与模型组比较,R50部位高、中剂量组小鼠ALT增强,差异有统计学意义(P<0.01或P<0.05),R50部位中剂量组小鼠肝脏系数升高,差异有统计学意义(P<0.05)。结论:何首乌水提物、R50部位及其主要成分对正常小鼠无明显急性肝毒性,但同样剂量的R50部位可能加剧荷肝癌小鼠的肝功能异常,在一定范围内呈剂量正相关。
OBJECTIVE： To study the acute hepatotoxicity effect of Polygonum multiflorum extract on normal and hepatocarcinoma-bearing mice. METHODS： Normal mice were divided into normal＇control group （constant volume 0.5 % sodium carboxymethylcellulose）, P. multiflorum total extract group （1 500 mg/kg） , R50 group （500 mg/kg） , 2, 3, 5, 4-tetrahydroxy stil- bene-2-O-β-d-glucoside group （TSG,- 185 mg/kg）, emodin-8-O-fl-d-glucoside group （EG, 2 mg/kg） ; they were given relevant medicines intragastrically once a day for consecutive 10 days. Hepatocarcinoma-bearing mice were divided into normal control group （constant volume 0.5 % sodium carboxymethylcellulose）, model group （constant volume 0.5 % sodium carboxymethylcellu- los）, R50 high-dose, medium-dose and low-dose groups （1 000, 500, 250 mg/kg） ; they were given relevant medicines intragastri- cally once a day for consecutive 10 days. The general conditions of mice were observed; and the activity of AST and ALT were de- termined; liver weight and body weight were also detected and liver coefficient was calculated. RESULTS： Compared with normal control group, general conditions, ALT, AST and liver coefficient had no obvious change in P. multiflorum total extract group, R50 group, TSG group and EG group（P〉0.05）. Compared with normal control group, AST, ALT and liver coefficient were in- creased in model group; there was statistical significance（P〈0.05）. Compared with model group, ALT of mice increased in R50 high-dose and medium-dose groups; there was statistical significance（P〈0.01 ,P〈0.05）. Liver coefficient were increased in R50 medium-dose group; there was statisitical significance （P〈0.05）. CONCLUSIONS： Water extract of P. multiflorum and its R50 part do not have obvious hepatoxicity on normal mice. However, the same dose of R50 may aggravate liver injury in hepatocarcino- ma-bearing mice, and have a dose-dependent manner within a certain range.
出处
《中国药房》
CAS
CSCD
2014年第15期1358-1361,共4页
China Pharmacy
基金
北京中医药大学自主选题资助项目(No.2013-JYBZZXS-088)
