摘要
目的：建立补中益气颗粒中黄芪甲苷的含量测定方法.方法：采用Diamonsil C18色谱柱（200 mm×4.6mm,5μm）,流动相为乙腈-水（35∶65）,流速为1.0 mL·min^-1,柱温30℃,蒸发光散射检测器,漂移管温度110℃,气体流速为3.0 L·min^-1.结果：黄芪甲苷浓度在2.682 ～21.456μg内线性关系良好（r=0.999 9）;平均回收率为98.3％,RSD=1.0％.结论：该方法灵敏、准确,可用于补中益气颗粒的质量控制.
Objective： To establish HPLC-ELSD method for determination of Astragaloside IV in Buzhong Yiqi Granules. Methods： Diamonsil C18 column（200 mm×4.6mm,5μm）was adopted and mobile phase consisted of acetonitrile-water （ 35 ： 65 ） . The flow rate was 1.0 mL· min^-1 . The column temperature is 30 ℃. The ELSD inlet heater was set at 110 ℃, and the flow rate of N2 is 3.0 L·min^-1 Results： The linear range was 2. 682- 21. 456 μg（ r = 0. 999 9）. The average recovery rate was 98.3% （ RSD = 1.0% ）. Conclusion： The method is sensitive, accurate, and can be used for the quality control of Buzhong Yicli Granules.
出处
《中国现代中药》
CAS
2014年第3期229-231,共3页
Modern Chinese Medicine
