摘要
以挤压-夹取法采集精荚,采用实验生态学、海水养殖学和个体标记的方法和技术研究捕自台湾海峡自然海区日本囊对虾(Marsupenaeus japonicus)成熟雄虾精荚多次再生的规律.结果表明:在水温(21±2)℃,盐度32±1,pH 8.1±0.2的条件下,初次成熟雄亲虾的体长或体质量与亲虾精荚质量之间呈正相关关系(p<0.01),与精荚中精子合格率、受精能力和精子总数之间的相关性不显著(p>0.05);雄亲虾在一个蜕壳周期内精荚可再生1~3次,平均为2次;精荚再生的时间间隔为5~7d,随精荚再生次数的递增,间隔时间有延长的态势,但差异不明显(p>0.05);精荚的质量、精子合格率和受精能力随再生次数增多呈下降趋势,差异明显(p<0.05);精子总数在不同批次间随再生次数增多总体呈上升趋势,差异显著(p<0.05),但在同一批次的再生精荚中精子总数呈现上下起伏波动.具有不同精荚再生能力的个体在亲虾群体中比例分布为:1次占87.5%,2次占80.6%,3次占44.4%.文中还就精荚不同获取方法对其再生的影响、雄虾性腺多次成熟能力的个体差异和再生精荚质量的问题等进行了详细的讨论,以期为日本囊对虾雄亲虾资源的利用和保护提供科学依据.
This research, using an integrated method of Extrusion-clip method, experimental ecology, mariculture and individual markings, was aimed at an investigation into the regularity of spermatophore regeneration of male shrimps, Marsupenaeus japoni- cus. The main results showed that： under the conditions of water temperature （214-2） ＊C, salinity 32 ~ 1, and pH 8.1 ~ 0.2, the spermatophore of the male broodstock had 3 times multi formation with an average of 2 times in one molt cycle; the interval is ranged from 5 days to 7 days, different batches had no significant differences （p^0. 05）; different batches of regenerated spermato- phore had a downward trend in weight and sperm fertilization rate, the difference was notable （p^0.05） ; but sperm count in differ- ent batches of regenerated spermatophore is ascent, moreover in the same batch of regenerated spermatophore sperm count had a thereabouts trend. The distribution of individuals with various regeneration ability in the population of male broodstock：all of the broodstock could generate one time,87.5 ~./0 two times, 80.6 % three times and 44.4 % four times. From the research, we can sum- marize that there are no significant differences between the size of the shrimps, M. japonicas and the quality of spermatophore.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第2期283-288,共6页
Journal of Xiamen University：Natural Science
基金
福建省科技计划重点项目(201203N30)
关键词
日本囊对虾
精荚
多次形成
再生能力
再生次数
Marsupenaeus japonicus
spermatophore
regeneration
regeneration ability
regeneration times
