摘要
建立了薄层色谱(TLC)与表面增强拉曼光谱(SERS)联用方法对降压类中药中四种非法添加的化学成分,即盐酸尼卡地平、甲磺酸多沙唑嗪、盐酸普萘洛尔及氢氯噻嗪进行快速检测的新方法。利用TLC法将掺杂成分与中药基质进行简单分离,采用SERS法对薄层板上的微量物质进行定性检测。实验中通过优化银胶浓度和薄层展开体系,并考察掺杂成分的最低检测限,初步建立可用于降压类中药非法添加化学成分的TLC-SERS检测方法。结果表明,该方法用于检测这四种化学成分时专属性较好,检测限低可至0.005μg,最终检测出十种降压类中药中有两种非法添加了化学成分。本方法简便快速,能实现降压类中药中非法添加化学成分的快速检测,有望成为中药掺杂化学成分的现场检测的新方法。
A novel facile method for on-site detection of antipertensive chemicals （e .g .nicardipine hydrochloride ,doxazosin me-sylate ,propranolol hydrochloride ,and hydrochlorothiazide） adulterated in traditional Chinese medicine for hypertension using thin layer chromatography （TLC） combined with surface enhanced Raman spectroscopy （SERS） was reported in the present pa-per .Analytes and pharmaceutical matrices was separated by TLC ,then SERS method was used to complete qualitative identifi-cation of trace substances on TLC plate .By optimizing colloidal silver concentration and developing solvent ,as well as exploring the optimal limits of detection （LOD） ,the initially established TLC-SERS method was used to detect real hypertension Chinese pharmaceuticals .The results showed that this method had good specificity for the four chemicals and high sensitivity with a limit of detection as lower as to 0.005μg .Finally ,two of the ten antipertensive drugs were detected to be adulterated with chemicals . This simple and fast method can realize rapid detection of chemicals illegally for doping in antipertensive Chinese pharmaceuti-cals ,and would have good prospects in on-site detection of chemicals for doping in Chinese pharmaceuticals .
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2014年第4期990-993,共4页
Spectroscopy and Spectral Analysis
基金
国家重大科学仪器专项项目(2012YQ180132)
上海市科委重点科技攻关项目(11431922502)资助
