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大鼠脊髓损伤模型Th17细胞分化中信号转导与转录激活因子3的作用 被引量：8

The role of signal transducer and activator of transcription 3 in Th17 cell differentiation in spinal cord injury rat model
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摘要 目的观察大鼠脊髓损伤模型中磷酸化信号转导与转录激活因子3(p-STAT3)及Th17相关因子的水平,初步探讨STAT3对脊髓损伤后Th17细胞的作用。方法成年健康雄性SD大鼠75只随机分为对照组(n=15)和模型组(每个时点n=15)。模型组采用改良Allen氏打击法建立脊髓损伤模型,对照组仅行椎板全切除术。在建模后1、24、48和72 h获取模型组大鼠损伤段脊髓、脾脏及外周血,免疫组织化学染色法检测白细胞介素(IL)-17在损伤段脊髓组织中的分布及表达情况,蛋白免疫印迹法检测p-STAT3在损伤段脊髓和脾脏组织中的表达变化,ELISA法检测外周血IL-6、IL-21和IL-23的动态变化,并与对照组大鼠作比较。结果模型组各时点IL-17、p-STAT3、IL-6、IL-21和IL-23的表达明显高于对照组(P<0.05),相关性分析显示p-STAT3的表达均与IL-17、IL-6及IL-21的表达呈正相关性(P<0.05)。结论 STAT3的异常激活可能是造成脊髓损伤后Th17细胞过度表达的重要原因。 Objective To investigate the expression of p -STAT3（signal transducer and activator of transcription 3） and Th17 cell related signal molecules in the rat model of spinal cord injury , so as to explore the roles of STAT3 and its related signal molecules in spinal cord injury .Methods A total of 75 adult male SD rats were randomly divided into two groups, control group （n=15） and model group （n=15 each time point）.Acute spinal cord injury rat model was estab-lished by the modified Allen weight drop method in the model group , while laminectomy alone was performed in control group.Each 15 rats in model group were sacrificed at the 1st, 24th, 48th and 72nd hour after operation for collection of spi-nal cord tissues, spleen tissues, and peripheral blood.Immunohistochemistry method was used to detect the expressions of interleukin （ IL）-17 in damaged spinal cord tissues , Western blot was applied to assess the protein expression of p -STAT3 in the spinal cord and spleen tissues , and ELISA was used to measure serum IL -6, IL-21 and IL-23.Results Expression of IL-17, p-STAT3, IL-6, IL-21 and IL-23 in model group was significantly higher than control group （P〈0.05）.Furthermore, the expression of p -STAT3 was significantly positively correlated with IL -17, IL-6 and IL-21 （P〈0.05）.Conclusion Aberrant activation of STAT3 may be one of factors to contribute to Th17 polariza-tion after spinal cord injury .
作者 宗少晖 曾高峰 方晔 邹斌 高太行
机构地区 广西医科大学第一附属医院脊柱骨病外科 广西医科大学公共卫生学院 广西医科大学研究生学院
出处 《广东医学》 CAS CSCD 北大核心 2014年第6期812-815,共4页 Guangdong Medical Journal
基金 广西高校科研项目(编号:201204LX063)
关键词 脊髓损伤 STAT3 白细胞介素-17 白细胞介素-6 白细胞介素-21 spinal cord injury signal transducer and activator of transcription 3 interleukin - 17 interleukin - 6 interleukin - 21
分类号 R737.9 [医药卫生—肿瘤]
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参考文献15

  • 1GUPTA R, BATHEN M E, SMITH J S, et al. Advances in the management of spinal cord injury [ J ]. J Am Acad Orthop Surg, 2010, 18(4) : 210 -222.
  • 2BAPTISTE D C, FEHLINGS M G. Pharmacological approaches to repair the injured spinal cord [ J ]. J Neurotrauma, 2006, 23 (3/ 4) : 318 -334.
  • 3SU X, YE J, HSUEH E C, et al. Tumor microenvironments direct the recruitment and expansion of human Thl7 cells[ J]. J Immu- nol, 2010, 184(3): 1630-1641.
  • 4罗健,李国忠.Th17细胞及其在神经系统疾病中的研究进展[J].免疫学杂志,2013,29(5):442-446. 被引量：5
  • 5ANKENY D P, POPOVICH P G. Mechanisms and implications of adaptive immune responses after traumatic spinal cord injury [ J ]. Neuroscience, 2009, 158(3): 1112-1121.
  • 6KORTYLEWSKI M, XIN H, KUJAWSHI M, et al. Regulation of the IL -23 and IL - 12 balance by Stat3 signaling in the tumor mi- croenvironment[ J]. Cancer Cell, 2009, 15(2) : 114 -123.
  • 7飞翔,锁志刚,丁惠强,唐晓杰,樊涛.JAK2/STAT3信号转导通路在大鼠急性脊髓损伤中对细胞凋亡的调控作用[J].中风与神经疾病杂志,2011,28(11):993-997. 被引量：5
  • 8KORTYLEWSKI M, XIN H, KUJAWSHI M, et al. Regulation of the IL -23 and IL - 12 balance by Stat3 Signaling in the tumor mi- croenvironment[ J]. Cancer Cell, 2009, 15(2) : 114 - 123.
  • 9WANG L, YI T, KORTYLEWSKI M, et al. IL- 17 can promote tumor growth through an IL - 6 - Stat3 signaling pathway [ J ]. J Exp Med, 2009, 206(7): 1457- 1464.
  • 10KWON M J, MA J, DING Y, et al. PKC -0 promotes Thl7 dif- ferentiation via up- regulation of Stat3 [ J]. J Immunol, 2012, 188(12) : 5887 -5897.

二级参考文献60

  • 1杨谦梓,雷翀,路志红,王百忍,熊利泽.JAK-STAT通路抑制剂和自由基清除剂联合应用对大鼠局灶性脑缺血/再灌注损伤的保护作用研究[J].中华危重病急救医学,2008,20(11). 被引量：8
  • 2Miyamoto M, Prause O, Sjostrand M, et al. Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways [J]. Immunol, 2003, 170(9):4665-4672.
  • 3Kolls JK, Linden A. Interleukin-17 family members and inflammation[J]. Immunity, 2004, 21(4): 467-476.
  • 4Crellin NK, Garcia RV, Hadisfar O, et al. Human CD4^+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4^+ CD25^+T regulatory cells[J]. J Immunol, 2005, 175(12): 8051-8059.
  • 5Peng G, Guo Z, Kiniwa Y, et al. Toll-like receptor 8- mediated reversal of CD4^+ regulatory T cell function [J]. Science, 2005, 309(5739): 1380-1384.
  • 6Yang L, Anderson DE, Baecher Allan C, et al. IL-21 and TGF-beta are required for differentiation of human T (H) 17 cells[J]. Nature, 2008, 454(7202): 350-352.
  • 7Korn T, Bettelli E, Gao W, et al. IL ,21 initiates an alternative pathway to induce proinflammatory T(H) 17 cells[J]. Nature, 2007, 448(7152): 484-487.
  • 8Ivanov II, MeKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17^+ T helper cells [J]. Cell, 2006, 126(6): 1121-1133.
  • 9Brustle A, Heink S, Huber M, et al. The development of inflammatory T(H)-17 cells requires interfer on-regulatory factor 4[J]. Nat Immunol, 2007, 8(9): 958-966.
  • 10Romagnani S. Human Thl7 cells [J]. Arthritis Res Ther, 2008, 10(2): 206 -213.

共引文献11

同被引文献76

  • 1张竹青,卢书明,陈美如,李春艳,刘丽娜,吕申.胃癌中STAT3、p-STAT3和Bcl-xL的表达及临床意义[J].肿瘤防治研究,2014,41(5):430-433. 被引量：9
  • 2刘念.特异酶促使淀粉样前体蛋白裂解成β-淀粉样蛋白[J].国外医学（药学分册）,2006,33(2):151-151. 被引量：1
  • 3Baptiste DC, Fehlings MG. Pharmacological approaches to repair the injured spinal cord [J]. J Neurotrauma, 2006, 23 (3 - 4) : 318-334.
  • 4Su X, Ye J, Hsueh EC, et al. Tumor microenvironments direct the re- cruitment and expansion of human Th 17 cells [J]. J Immunol, 2010, 184(3) : 1630-1641.
  • 5Hu JZ, Huang JH, Xiao ZM, et al. Tetramethylpyrazine accelerates the function recovery of traumatic spinal cord in rat model by atten- uating inflammation [ J ]. J Neurol Sci, 2013,324 ( 1-2 ) : 94-99.
  • 6Schwartz M, Lazarov-Spiegler O, Rapalino O, et al. Potential repair of rat spinal cord injuries using stimulated homologous macro- phages[J]. Neurosurgery, 1999,44(5): 1041-1045.
  • 7Popovich PG, Wei P, Stokes BT. Cellular inflammatory response af- ter spinal cord injury in Sprague- Dawley and Lewis rats [J]. J Comp Neurol, 1997,377 (3) : 443-464.
  • 8McAllister F, Henry A, Kreindler JL, et al. Role of IL-17A, IL-17F, and the IL-17 receptor in regulating growth-related oncogene-alpha and granulocyte colony stimulating factor in bronchial epithelium: implications for airway inflammation in cystic fibrosis [J]. J Immu- nol,2005,175( 1 ) :404-412.
  • 9Steinman L. A brief history of T(H)17, the first major revision in the T (H) 1/T (H)2 hypothesis of T cell-mediated tissue damage [J]. Nat Med,2007,13(2) : 139-145.
  • 10Kortylewski M, Xin H, Kujawski M, et al. Regulation of the IL-23 and IL-12 balance by Stat3 signaling in the tumor microenviron- ment[J]. Cancer Ce11,2009,15(2) : 114-123.

引证文献8

二级引证文献23

广东医学
2014年 第6期

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