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HPLC同时测定栀子金花丸中4种成分的含量 被引量:2

Simultaneous Determination of 4 Components in Zhizijinhua Pill by HPLC
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摘要 [目的]采用HPLC建立同时测定栀子金花丸中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素这4种成分含量的分析方法.[方法]色谱柱为Agilent Zorbax SB-C18(4.6 mm×250 mm,5 μm);流动相为乙腈-浓度0.1%磷酸溶液,梯度洗脱,检测波长为278 nm.[结果]黄芩苷、汉黄芩苷、黄芩素、汉黄芩素4种成分的线性范围分别为0.418 ~4.18 μg/ml(R =0.999 8),0.2~2.0 μg/ml(R =0.999 8),0.233~2.330 μg/ml(R =0.999 9),0.215 ~2.150 μg/ml(R =0.999 9);平均回收率分别是98.40% (RSD =0.45%)、98..66%(RSD=1.41%)、98.88%(RSD=1.48%)和98.97% (RSD=1.45%).[结论]该方法快速、简便,且具有良好的重复性和回收率,可作为栀子金花丸中这4种成分的含量控制方法. [Objective] To establish a method for the simultaneous determination of Baicalin,Wogonoside,Baicalein and Wogonin in Zhizijinhua Pill using HPLC.[Method] The separation was carried out on a Agilent Zorbax SB-C_(18)(4.6 mm × 250 mm,5μm).The mobile phase was methanol-0.1% phosphoric acid with gradient elution and 278nm wavelength detection.[Result] The Baicalin,Wogonoside,Baicalein,Wogonin had good linearity in the ranges of 0.418-4.18 μg/ml (R =0.999 8),0.2-2 μg/ml (R =0.999 8),0.233-2.330μg/ml(R =0.999 9) and 0.215-2.150 μg/ml(R =0.999 9).The average recoveries were 98.40% (RSD =0.45%),98.66% (RSD =1.41%),98.88 % (RSD =1.48 %),98.97 % (RSD =1.45 %),respectively.[Conclusion] This method is rapid,simple with good repeatability and recovery,so it can be used as a quantitative analysis method for Zhizijinhua Pill.
出处 《安徽农业科学》 CAS 2014年第6期1665-1666,共2页 Journal of Anhui Agricultural Sciences
关键词 栀子金花丸 HPLC 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 Zhizijinhua Pill HPLC Baicalin Wogonoside Baicalein Wogonin
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