摘要
DNA的烷基化损伤可导致复制过程中的错配,被认为是引起基因突变及相关疾病的原因。烷基化DNA加合物是重要的DNA烷基化损伤产物。本文介绍了DNA烷基化损伤的修复机制以及产物;综述了烷化类损伤试剂,及其作用于细胞产生烷基化DNA加合物的机理;讨论了烷化类DNA加合物与吸烟之间的关系;综述了烷化类DNA加合物的检测方法;展望了烷基化DNA加合物的研究方向及作为烷基化损伤标志物的研究前景。
The fact that DNA alkylation damage might lead to mismatch in replication was considered as cause of gene mutation and other relevant disease. The variety of DNA alkylation damage, repair mechanisms and its products,the generative mechanism in vivo and analytical method of alkylation DNA adducts were reviewed. Current analytical methods of alkylation DNA adducts include Immunoaffinity Chromatography/32P-postlabelling technique （IC-32P）, Gas Chromatogeraph-Mass Spectrometer （GC-MS） and Liquid Chromatography-tandem Mass Spectrometer （LC-MS/MS）. IC-32P had excellent performance on sensibility, but it took many complicated steps in determining alkylation DNA adducts. The sample usually cost more in the method of GC-MS during pre-handling process as derivatization is needed;LC-MS/MS method offered many practical advantages in pre-handling such as stability, selectivity and sensibility, which can enhance the efficiency of sample analysis. As biomarkers of DNA alkylation damage, alkylation DNA adducts played a significant role in risk evaluation of alkylation reagents.
出处
《中国烟草学报》
EI
CAS
CSCD
北大核心
2014年第1期96-102,共7页
Acta Tabacaria Sinica
